Bombyx mori transcription factors FoxA and SAGE divergently regulate the expression of wing cuticle protein gene 4 during metamorphosis
Bombyx mori transcription factors FoxA and SAGE divergently regulate the expression of wing cuticle protein gene 4 during metamorphosis
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家蚕转录因子FoxA和SAGE在变态过程中不同程度地调控翅角质层蛋白基因4的表达
DOI：
10.1074/jbc.ra118.004395
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发表时间：
2018-11
期刊：
影响因子：
--
通讯作者：
Qili Feng
中科院分区：
文献类型：
--
作者：
Qihao Hu;Zidan Zhu;Danhui Zhao;Baojuan Zeng;Sichun Zheng;Qisheng Song;Huimin Deng;Qili Feng
家蚕变态的阶段特异性基因表达。B。家蚕翅角质层蛋白基因4（BmWCP 4）是一个在蛹期特异表达的翅盘发育必需基因。BmWCP 4转录抑制在幼虫阶段的未知机制，我们试图在这里阐明。生物信息学分析预测了BmWCP 4启动子区域中7个潜在的叉头盒（Fox）顺式调节元件（克雷斯），我们发现Fox CRE 6有助于抑制BmWCP 4的表达。电泳迁移率变动（EMSA）和DNA下拉分析显示，BmFoxA抑制活性的BmWCP 4启动子特异性结合的福克斯CRE 6。BmFoxA在幼虫期翅盘中的表达量高于蛹期。相反，另一种转录因子BmSAGE的表达在发育过程中增加。值得注意的是，控制昆虫蜕皮的激素20-羟基蜕皮酮（20 E）抑制了翼盘中BmFoxA的表达，并上调了BmSage的表达。EMSA和细胞共转染试验表明BmSAGE与BmFoxA相互作用并抑制其与Fox CRE 6的结合，从而释放BmFoxA介导的BmWCP 4抑制。总之，在幼虫阶段较高的BmFoxA表达通过与BmWCP 4启动子上的Fox CRE 6结合来抑制BmWCP 4表达。在变态过程中，BmSAGE与BmFoxA形成复合物以解除这种抑制，启动BmWCP 4表达。综上所述，本研究揭示了BmFoxA在调节BmWCP 4表达中的开关样作用，并为昆虫翅盘发育的调控提供了新的见解。
Stage-specific gene expression governs metamorphosis of the silkworm, Bombyx mori. B. mori wing cuticle protein gene 4 (BmWCP4) is an essential gene for wing disc development expressed specifically during pupation. BmWCP4 transcription is suppressed at the larval stage by unknown mechanisms, which we sought to elucidate here. Bioinformatics analysis predicted seven potential Forkhead box (Fox) cis-regulatory elements (CREs) in the BmWCP4 promoter region, and we found that Fox CRE6 contributes to suppression of BmWCP4 expression. Electrophoretic mobility shift (EMSA) and DNA pull-down assays revealed that BmFoxA suppressed activity at the BmWCP4 promoter by specifically binding to the Fox CRE6. The expression level of BmFoxA in the wing discs was higher during the larval stage than at the pupal stage. In contrast, expression of another transcription factor, BmSAGE, increased over the course of development. Of note, the hormone 20-hydroxyecdysone (20E), which governs molting in insects, suppressed BmFoxA expression in the wing discs and up-regulated that of BmSage. EMSA and cell co-transfection assays indicated that BmSAGE interacted with BmFoxA and suppressed its binding to the Fox CRE6, thereby releasing BmFoxA-mediated suppression of BmWCP4. In summary, higher BmFoxA expression during the larval stage suppresses BmWCP4 expression by binding to the Fox CRE6 on the BmWCP4 promoter. During metamorphosis, BmSAGE forms a complex with BmFoxA to relieve this repression, initiating BmWCP4 expression. Taken together, this study reveals a switchlike role for BmFoxA in regulating BmWCP4 expression and provides new insights into the regulatory regulation of wing disc development in insects.
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影响因子：
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