Optically transparent recombinant silk-elastinlike protein polymer films.
Optically transparent recombinant silk-elastinlike protein polymer films.
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DOI：
10.1021/jp109764f
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发表时间：
2011-02-24
期刊：
影响因子：
--
通讯作者：
Wu X
中科院分区：
文献类型：
--
作者：
Teng W;Huang Y;Cappello J;Wu X
重组蛋白聚合物被广泛评价为在药物输送和组织工程中应用的生物材料，但很少有报道是光学透明的。在这里，我们报告了由基因工程丝状弹性蛋白聚合物SELP-47K组成的薄膜的显著光学透明度。厚度为100μm的SELP-47K薄膜在350800 nm波长范围内的透过率为93%。以戊二醛为交联剂的SEP-47K在800 nm波长处的透光率为77%，而以甲醇为交联剂的非共价交联剂的透光率仅为95%。SELP-47K薄膜的非共价和共价交联也影响其二级结构和水分含量。细胞存活率和增殖分析进一步表明，非和共价交联型SELP-47K膜具有良好的细胞相容性。SELP-47K薄膜的高光学透明性和细胞相容性，以及以前报道的优异的机械性能，表明这种蛋白质聚合物可能在独特的、新的生物医学应用中有用。
Recombinant protein polymers, evaluated extensively as biomaterials for applications in drug delivery and tissue engineering, are rarely reported as being optically transparent. Here we report the notable optical transparency of films composed of a genetically engineered silk-elastinlike protein polymer SELP-47K. SELP-47K films of 100 μm in thickness display a transmittance of 93% in the wavelength range of 350–800 nm. While covalent cross-linking of SELP-47K via glutaraldehyde decreases its transmittance to 77% at the wavelength of 800 nm, noncovalent cross-linking using methanol slightly increases it to 95%. Non- and covalent cross-linking of SELP-47K films also influences their secondary structures and water contents. Cell viability and proliferation analyses further reveal the excellent cytocompatibility of both non- and covalently cross-linked SELP-47K films. The combination of high optical transparency and cytocompatibility of SELP-47K films, together with their previously reported outstanding mechanical properties, suggests that this protein polymer may be useful in unique, new biomedical applications.
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