Silk film biomaterials for cornea tissue engineering.
Silk film biomaterials for cornea tissue engineering.
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DOI：
10.1016/j.biomaterials.2008.11.018
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发表时间：
2009-03
期刊：
影响因子：
14
通讯作者：
Kaplan DL
中科院分区：
文献类型：
--
作者：
Lawrence BD;Marchant JK;Pindrus MA;Omenetto FG;Kaplan DL
用于角膜组织工程的生物材料必须在体内具有潜在应用的几个关键特性，包括透明度、机械完整性、生物相容性和缓慢的生物降解性。丝膜生物材料经过设计和表征以满足这些功能要求。丝蛋白膜被用于一种仿生方法来复制角膜基质组织结构。这些膜厚2μm，以模拟角膜胶原薄片的尺寸，并进行表面图案化以引导细胞排列。为了增强营养物质的跨层扩散并促进细胞间相互作用，在丝膜中引入了直径为0.5到5.0μm的孔隙。在二维和三维培养中，都证明了人和兔角膜成纤维细胞在这些膜上的增殖、排列以及角膜细胞外基质的表达。这些膜的机械性能、光学透明度和表面图案化特征，以及它们支持角膜细胞功能的能力表明，这种新的生物材料系统为角膜组织再生提供了重要的潜在益处。
Biomaterials for corneal tissue engineering must demonstrate several critical features for potential utility in vivo, including transparency, mechanical integrity, biocompatibility and slow biodegradation. Silk film biomaterials were designed and characterized to meet these functional requirements. Silk protein films were used in a biomimetic approach to replicate corneal stromal tissue architecture. The films were 2 μm thick to emulate corneal collagen lamellae dimensions, and were surface patterned to guide cell alignment. To enhance trans-lamellar diffusion of nutrients and to promote cell-cell interaction, pores with 0.5 to 5.0 μm diameters were introduced into the silk films. Human and rabbit corneal fibroblast proliferation, alignment and corneal extracellular matrix expression on these films in both 2D and 3D cultures was demonstrated. The mechanical properties, optical clarity and surface patterned features of these films, combined with their ability to support corneal cell functions suggest this new biomaterial system offers important potential benefits for corneal tissue regeneration.
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影响因子：
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