Biosynthetic potential-based strain prioritization for natural product discovery: a showcase for diterpenoid-producing actinomycetes.
Biosynthetic potential-based strain prioritization for natural product discovery: a showcase for diterpenoid-producing actinomycetes.
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DOI：
10.1021/np401063s
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发表时间：
2014-02-28
影响因子：
5.1
通讯作者：
Shen B
中科院分区：
文献类型：
--
作者：
Xie P;Ma M;Rateb ME;Shaaban KA;Yu Z;Huang SX;Zhao LX;Zhu X;Yan Y;Peterson RM;Lohman JR;Yang D;Yin M;Rudolf JD;Jiang Y;Duan Y;Shen B
天然产物仍然是药物和药物先导的最佳来源，并作为优秀的小分子探针来剖析基本的生物过程。天然产物社区面临的一个巨大挑战是有效地和成本有效地发现新的天然产物。在这里，我们报告了一种实用的方法来调查微生物中的生物合成潜力，从而确定最有前途的菌株，并优先考虑它们的天然产物发现。我们的方法的核心是创新性的制备，通过两层PCR方法，一个路径特异性探针池，从而允许调查目标类别的天然产物的生物合成机制的所有变体。该方法的效用被证明通过调查100株，随机选择从我们的放线菌收集，为他们的生物合成潜力的四类天然产物，芳香聚酮，减少聚酮，非核糖体肽，和二萜类化合物，确定16人才菌株。最终选择了其中一种有才华的菌株，灰色链霉菌CB 00830，以展示目标天然产物类别的发现，从而分离出三种二萜类化合物，六种非核糖体肽和相关代谢物以及三种聚酮化合物。这种方法的变化应适用于发现其他类别的天然产物。
Natural products remain the best sources of drugs and drug leads and serve as outstanding small-molecule probes to dissect fundamental biological processes. A great challenge for the natural product community is to discover novel natural products efficiently and cost effectively. Here we report the development of a practical method to survey biosynthetic potential in microorganisms, thereby identifying the most promising strains and prioritizing them for natural product discovery. Central to our approach is the innovative preparation, by a two-tiered PCR method, of a pool of pathway-specific probes, thereby allowing the survey of all variants of the biosynthetic machineries for the targeted class of natural products. The utility of the method was demonstrated by surveying 100 strains, randomly selected from our actinomycete collection, for their biosynthetic potential of four classes of natural products, aromatic polyketides, reduced polyketides, nonribosomal peptides, and diterpenoids, identifying 16 talented strains. One of the talented strains, Streptomyces griseus CB00830, was finally chosen to showcase the discovery of the targeted classes of natural products, resulting in the isolation of three diterpenoids, six nonribosomal peptides and related metabolites, and three polyketides. Variations of this method should be applicable to the discovery of other classes of natural products.
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影响因子：
7.4
作者：
Konishi, Yasuo;Kiyota, Taira;Purisima, Enrico
通讯作者：
Purisima, Enrico
影响因子：
15
作者：
Kwon, HJ;Smith, WC;Shen, B
通讯作者：
Shen, B
DOI：
10.1073/pnas.88.5.1878
发表时间：
1991-03-01
影响因子：
11.1
作者：
ANKENBAUER, RG;STALEY, AL;COX, CD
通讯作者：
COX, CD
影响因子：
11.9
作者：
Nett M;Ikeda H;Moore BS
通讯作者：
Moore BS
深入了解硫链丝菌素生物合成中喹哪二酸部分的形成，促进氟化硫肽的生成
影响因子：
--
作者：
Duan, Lian;Wang, Shoufeng;Liu, Wen
通讯作者：
Liu, Wen