Galactosyl ceramide (or a closely related molecule) is the receptor for human immunodeficiency virus type 1 on human colon epithelial HT29 cells
Galactosyl ceramide (or a closely related molecule) is the receptor for human immunodeficiency virus type 1 on human colon epithelial HT29 cells
复制标题
半乳糖基神经酰胺（或密切相关的分子）是人结肠上皮 HT29 细胞上 1 型人类免疫缺陷病毒的受体
作者：
N. Yahi;S. Baghdiguian;Herve Moreau;Jacques FANTINIlt;N. Yahi
胃肠道被认为是人类免疫缺陷病毒(HIV)的主要感染途径。HIV对人类结肠上皮细胞的感染不能被已知的阻止淋巴细胞感染的抗CD4抗体阻止(J.Fantini，N.Yahi和J.C.Chermann，Proc。娜塔莉。阿卡德。SCI。美国88：9297-9301,1991)，表明这些细胞上存在HIV的替代受体。在这篇报道中，我们发现(I)针对半乳糖神经酰胺的单抗抑制了两种截然不同的HIV-1毒株对HT29细胞的感染；(Ii)通过免疫荧光和电子显微镜免疫定位，该抗体强烈标记了HT29细胞的表面；(Iii)标记优先但不完全限于分化的结肠细胞的基侧膜域，这与HIV感染这些上皮细胞的顶面和基面的能力一致；以及(Iv)在HT29细胞制备的中性糖脂的薄层层析免疫染色实验中，抗体与对应于半乳糖神经酰胺的神经酰胺单糖苷组分特异地反应。我们没有在淋巴细胞中检测到这种糖脂，而且抗半乳糖神经酰胺抗体始终无法抑制这些细胞对HIV的感染。这些数据表明，半乳糖基神经酰胺(或其衍生物)是HT29细胞表面HIV受体的重要组成部分。
The gastrointestinal tract is considered to be a major route of infection for human immunodeficiency virus (HIV). Infection of human colon epithelial cells by HIV is not blocked by anti-CD4 antibodies known to block infection of lymphoid cells (J. Fantini, N. Yahi, and J. C. Chermann, Proc. Natl. Acad. Sci. USA 88:9297-9301, 1991), suggesting the presence of an alternate receptor for HIV on these cells. In this report, we show that (i) a monoclonal antibody specifically directed against galactosyl ceramide inhibited the infection of HT29 cells by two markedly different strains of HIV-1, as assessed by polymerase chain reaction amplification and reverse transcriptase assay; (ii) this antibody strongly labeled the surface of HT29 cells by immunofluorescence and electron microscopic immunolocalization; (iii) the labeling was preferentially but not totally restricted to the basolateral membrane domain of differentiated colonic cells, in agreement with the ability of HIV to infect both the apical and basolateral surfaces of these epithelial cells; and (iv) in thin-layer chromatography-immunostaining experiments with neutral glycolipids prepared from HT29 cells, the antibody specifically reacted with a ceramide monoglycoside fraction corresponding to galactosyl ceramide. We did not detect this glycolipid in lymphoid cells, and anti-galactosyl ceramide antibodies consistently failed to inhibit HIV infection of these cells. These data suggest that galactosyl ceramide (or a derivative) is an essential component of the receptor for HIV on the surface of HT29 cells.
影响因子：
56.9
作者：
HAROUSE, JM;BHAT, S;GONZALEZSCARANO, F
通讯作者：
GONZALEZSCARANO, F
胰蛋白酶灭活 HIV-1 及其在证明细胞的特定病毒感染中的应用。
影响因子：
3.1
作者：
Tang,SB;Levy,JA
通讯作者：
Levy,JA
影响因子：
--
作者：
S. Hakomori
通讯作者：
S. Hakomori