Identification of rare microbial colonizers of plastic materials incubated in a coral reef environment.
Identification of rare microbial colonizers of plastic materials incubated in a coral reef environment.
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DOI：
10.3389/fmicb.2023.1259014
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发表时间：
2023
影响因子：
5.2
通讯作者：
中科院分区：
文献类型：
--
作者：
海洋环境中塑料垃圾的堆积对生态产生了复杂的、意外的影响，这些影响跨越了社区组织的各个层面。为了测量聚烯烃定植的海洋细菌群落的演替，在百慕大平台的低营养海岸水域进行了现场时间序列实验。我们的目标是鉴定聚烯烃定植类群，并分离细菌培养物，以便将来研究微生物-塑料相互作用的生物化学。将高密度聚乙烯、低密度聚乙烯、聚丙烯和玻璃片在表层海水中孵育11 周，每隔两周取样一次。用16S rDNA测序和ATR-FTIR/HIM分析了生物膜群落结构和聚合物表面的化学变化。主要的定殖性分类群是以前报道的海洋环境中表面的世界性定殖者，不同类型的塑料之间高度相似。然而，在不同塑料类型之间观察到稀有群落组成的显著差异，这可能表明基于表面化学的特定相互作用。出乎意料的是，在第42天和56天之间，所有物质处理的群落组成发生了重大转变(p <； 0.01)。过渡期前的优势定殖者为Alteromonadaceae、Marinomonadaceae、SaccharSpirillaceae、Vibrionaceae、Thalassspiraceae和黄杆菌科。过渡后，这些类群的相对丰度下降，而水生单胞菌科、红杆菌科和腐螺旋科的相对丰度增加。在培养过程中，观察到8,641个定植分类群，其中25个显著富含特定的聚烯烃。已知的7个富集族包括碳氢化合物降解菌(Hyphomonadaceae、Parugarculaceae和Rhodbacteraceae)和1个正构烷烃降解菌(Ketbacter sp.)。表现出与特定聚烯烃相关的ASV是正在进行的旨在回收培养中的塑料降解微生物的研究的目标。
Plastic waste accumulation in marine environments has complex, unintended impacts on ecology that cross levels of community organization. To measure succession in polyolefin-colonizing marine bacterial communities, an in situ time-series experiment was conducted in the oligotrophic coastal waters of the Bermuda Platform. Our goals were to identify polyolefin colonizing taxa and isolate bacterial cultures for future studies of the biochemistry of microbe-plastic interactions. HDPE, LDPE, PP, and glass coupons were incubated in surface seawater for 11 weeks and sampled at two-week intervals. 16S rDNA sequencing and ATR-FTIR/HIM were used to assess biofilm community structure and chemical changes in polymer surfaces. The dominant colonizing taxa were previously reported cosmopolitan colonizers of surfaces in marine environments, which were highly similar among the different plastic types. However, significant differences in rare community composition were observed between plastic types, potentially indicating specific interactions based on surface chemistry. Unexpectedly, a major transition in community composition occurred in all material treatments between days 42 and 56 (p < 0.01). Before the transition, Alteromonadaceae, Marinomonadaceae, Saccharospirillaceae, Vibrionaceae, Thalassospiraceae, and Flavobacteriaceae were the dominant colonizers. Following the transition, the relative abundance of these taxa declined, while Hyphomonadaceae, Rhodobacteraceae and Saprospiraceae increased. Over the course of the incubation, 8,641 colonizing taxa were observed, of which 25 were significantly enriched on specific polyolefins. Seven enriched taxa from families known to include hydrocarbon degraders (Hyphomonadaceae, Parvularculaceae and Rhodobacteraceae) and one n-alkane degrader (Ketobacter sp.). The ASVs that exhibited associations with specific polyolefins are targets of ongoing investigations aimed at retrieving plastic-degrading microbes in culture.
影响因子：
3.7
作者：
Davis, Edward W.;Wong, Carmen P.;Arnold, Holly K.;Kasschau, Kristin;Gaulke, Christopher A.;Sharpton, Thomas J.;Ho, Emily
通讯作者：
Ho, Emily
影响因子：
4.3
作者：
Sharpton TJ;Riesenfeld SJ;Kembel SW;Ladau J;O'Dwyer JP;Green JL;Eisen JA;Pollard KS
通讯作者：
Pollard KS