Age and micronutrient effects on the microbiome in a mouse model of zinc depletion and supplementation.
Age and micronutrient effects on the microbiome in a mouse model of zinc depletion and supplementation.
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DOI：
10.1371/journal.pone.0275352
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发表时间：
2022
期刊：
影响因子：
3.7
通讯作者：
Ho, Emily
中科院分区：
文献类型：
--
作者：
Davis, Edward W.;Wong, Carmen P.;Arnold, Holly K.;Kasschau, Kristin;Gaulke, Christopher A.;Sharpton, Thomas J.;Ho, Emily
老年人有缺锌的风险，缺锌可能使他们易患免疫功能障碍和与年龄相关的慢性炎症，从而导致无数疾病和病症。最近的研究还表明，肠道微生物组与年龄相关性炎症的发生和严重程度有关，这表明饮食锌状态和肠道微生物组可能相互作用，影响与年龄相关的宿主免疫力。我们假设，肠道微生物组中与年龄相关的改变有助于宿主锌水平的锌缺乏和炎症增加。我们在C57 BL/6小鼠模型中采用多因素两部分研究设计来验证这一假设。这两项研究包括分别饲喂（1）锌充足或锌补充饲料或（2）锌充足或锌微量缺乏饲料的幼龄（2个月大）和老龄（24个月大）小鼠。总体微生物组组成并没有随着锌状态发生显著变化; β多样性几乎完全由年龄效应驱动。年龄导致的微生物组差异在所有分类水平上都很明显，超过一半的分类单位存在显著差异。此外，我们发现186个属中有150个属在两个年龄组之间存在显著差异，拟杆菌和副拟杆菌分别是年轻和老年小鼠的主要类群。这些数据表明，调节单个微量营养素浓度不会导致全面的微生物组变化，而是影响肠道微生物组的特定组分。然而，系统发育聚集技术（ClaaTU）揭示了系统发育分支，以年龄依赖性的方式对饮食锌状态和炎症状态的调节作出反应。总的来说，这些结果表明，宿主年龄，肠道微生物组组成和饮食锌状态之间存在复杂的相互作用。
Older adult populations are at risk for zinc deficiency, which may predispose them to immune dysfunction and age-related chronic inflammation that drives myriad diseases and disorders. Recent work also implicates the gut microbiome in the onset and severity of age-related inflammation, indicating that dietary zinc status and the gut microbiome may interact to impact age-related host immunity. We hypothesize that age-related alterations in the gut microbiome contribute to the demonstrated zinc deficits in host zinc levels and increased inflammation. We tested this hypothesis with a multifactor two-part study design in a C57BL/6 mouse model. The two studies included young (2 month old) and aged (24 month old) mice fed either (1) a zinc adequate or zinc supplemented diet, or (2) a zinc adequate or marginal zinc deficient diet, respectively. Overall microbiome composition did not significantly change with zinc status; beta diversity was driven almost exclusively by age effects. Microbiome differences due to age are evident at all taxonomic levels, with more than half of all taxonomic units significantly different. Furthermore, we found 150 out of 186 genera were significantly different between the two age groups, with Bacteriodes and Parabacteroides being the primary taxa of young and old mice, respectively. These data suggest that modulating individual micronutrient concentrations does not lead to comprehensive microbiome shifts, but rather affects specific components of the gut microbiome. However, a phylogenetic agglomeration technique (ClaaTU) revealed phylogenetic clades that respond to modulation of dietary zinc status and inflammation state in an age-dependent manner. Collectively, these results suggest that a complex interplay exists between host age, gut microbiome composition, and dietary zinc status.
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影响因子：
5.9
作者：
Badal VD;Vaccariello ED;Murray ER;Yu KE;Knight R;Jeste DV;Nguyen TT
通讯作者：
Nguyen TT
影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
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影响因子：
12.3
作者：
Debelius J;Song SJ;Vazquez-Baeza Y;Xu ZZ;Gonzalez A;Knight R
通讯作者：
Knight R
DOI：
10.1126/science.1198719
发表时间：
2011-05-20
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Muegge BD;Kuczynski J;Knights D;Clemente JC;González A;Fontana L;Henrissat B;Knight R;Gordon JI
通讯作者：
Gordon JI
DOI：
10.1073/pnas.1419341112
发表时间：
2015-07-07
影响因子：
11.1
作者：
O'Dwyer, James P.;Kembel, Steven W.;Sharpton, Thomas J.
通讯作者：
Sharpton, Thomas J.