Biomimetic remineralization as a progressive dehydration mechanism of collagen matrices--implications in the aging of resin-dentin bonds.
Biomimetic remineralization as a progressive dehydration mechanism of collagen matrices--implications in the aging of resin-dentin bonds.
复制标题
DOI：
10.1016/j.actbio.2010.03.021
复制
发表时间：
2010-09
影响因子：
9.7
通讯作者：
Tay, Franklin R.
中科院分区：
文献类型：
--
作者：
Kim, Young Kyung;Mai, Sui;Mazzoni, Annalisa;Liu, Yan;Tezvergil-Mutluay, Arzu;Takahashi, Kei;Zhang, Kai;Pashley, David H.;Tay, Franklin R.
生物矿化是一种脱水过程，在这个过程中，胶原纤维内的水逐渐被磷灰石取代。由于水是导致树脂-牙本质粘结缺乏耐久性的重要因素，本研究考察了仿生再矿化策略作为一种渐进脱水机制的使用，以保持关节完整性和老化后的粘结强度。人牙本质表面用牙本质粘结剂粘接，用树脂复合体修复，然后切成含有粘接接头的棒状。实验标本在含有仿生类似物的再矿化介质中老化，对照组标本在模拟体液中老化长达12个月。从指定的时间段取出的标本通过使用银示踪剂的透射电子显微镜检查富水区域的表现以及粘连关节内的胶原降解情况。进行拉伸测试，以确定老化后粘结完整性的潜在损失。对照标本老化后粘接关节内的胶原蛋白严重降解。再矿化标本表现为进行性脱水，表现为银示踪剂减少和粘连关节内充满水的微通道的部分再矿化，以及胶原纤维内的再矿化，作为粘接过程的一部分，胶原纤维最初被脱矿。仿生再矿化作为富含水分、疏松树脂的胶原基质的一种渐进脱水机制，使这些粘合接头能够在12个月的老化过程中抵抗降解，这一点通过其拉伸粘合强度的保持得到了验证。概念验证仿生再矿化策略防止粘结降解的能力保证了临床相关递送系统的进一步发展。
Biomineralization is a dehydration process in which water from the intrafibrillar compartments of collagen fibrils are progressively replaced by apatites. As water is an important element that precipitates the lack of durability of resin-dentin bonds, this study examined the use of a biomimetic remineralization strategy as a progressive dehydration mechanism for preserving joint integrity and maintaining adhesive strength after aging. Human dentin surfaces were bonded with dentin adhesives, restored with resin composites and sectioned into sticks containing the adhesive joint. Experimental specimens were aged in a biomimetic analog-containing remineralizing medium and control specimens in simulated body fluid for up to 12 months. Specimens retrieved from the designated periods were examined by transmission electron microscopy for manifestation of water-rich regions using a silver tracer and for collagen degradation within the adhesive joints. Tensile testing was performed to determine the potential loss of bond integrity after aging. Control specimens exhibited severe collagen degradation within the adhesive joint after aging. Remineralized specimens exhibited progressive dehydration as manifested by silver tracer reduction and partial remineralization of water-filled micro-channels within the adhesive joint, as well as intrafibrillar remineralization of collagen fibrils that were demineralized initially as part of the bonding procedure. Biomimetic remineralization as a progressive dehydration mechanism of water-rich, resin-sparse collagen matrices enables those adhesive joints to resist degradation over the 12-month aging period, as verified by the conservation of their tensile bond strengths. The ability of the proof-of-concept biomimetic remineralization strategy to prevent bond degradation warrants further development of clinically-relevant delivery systems.
登录
查看更多内容
影响因子：
3.8
作者：
Deshpande, Atul S.;Beniash, Elia
通讯作者：
Beniash, Elia
影响因子：
3.4
作者：
Chesnick, Ingrid E.;Mason, Jeffrey T.;Potter, Kimberlee
通讯作者：
Potter, Kimberlee
影响因子：
3
作者：
Mjor, IA;Nordahl, I
通讯作者：
Nordahl, I
影响因子：
5
作者：
Reis, A.;Grande, R. H. M.;Loguerico, A. D.
通讯作者：
Loguerico, A. D.
DOI：
10.1002/jbm.b.31295
发表时间：
2009-07
影响因子：
3.4
作者：
Carrilho, Marcela R.;Tay, Franklin R.;Donnelly, Adam M.;Agee, Kelli A.;Tjaederhane, Leo;Mazzoni, Annalisa;Breschi, Lorenzo;Foulger, Stephen;Pashley, David H.
通讯作者：
Pashley, David H.