Host-derived loss of dentin matrix stiffness associated with solubilization of collagen.
Host-derived loss of dentin matrix stiffness associated with solubilization of collagen.
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DOI：
10.1002/jbm.b.31295
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发表时间：
2009-07
影响因子：
3.4
通讯作者：
Pashley, David H.
中科院分区：
文献类型：
--
作者：
Carrilho, Marcela R.;Tay, Franklin R.;Donnelly, Adam M.;Agee, Kelli A.;Tjaederhane, Leo;Mazzoni, Annalisa;Breschi, Lorenzo;Foulger, Stephen;Pashley, David H.
结合到牙本质基质的基质金属蛋白酶（MMP）在粘合剂粘结过程中被激活，并且被认为有助于树脂-牙本质粘结随时间的进行性降解。本研究的目的是评价脱矿牙本质的机械，生化和结构性能的变化与洗必泰（CHX），一种已知的MMP-抑制剂处理。在EDTA或磷酸（PA）中对牙本质梁进行脱矿处理后，通过三点弯曲测量每个梁的基线弹性模量（E）。用水（对照）或2%CHX（实验）预处理样本，然后在人工唾液（AS）中于37°C孵育4周。每周重新测量每个样本的E，并在孵育的第一周和第四周分析培养基中溶解的牙本质胶原。在脱矿后和孵育4周后立即对一些标本进行电子显微镜（TEM）检查。在EDTA和PA-脱矿物质标本中，对照标本的E在AS中孵育后下降（p<0.05），而CHX预处理标本的E随时间推移没有变化。1周或4周后，PA-脱矿物质对照（p<0.05）比EDTA-脱矿物质基质溶解更多的胶原。与PA相比，从CHX预处理的在EDTA中脱矿物质的标本中溶解的胶原蛋白较少（p<0.05）。对照梁的TEM检查显示，在10%PA（12小时）中的牙本质长期脱矿并没有使胶原纤维变性。
Matrix metalloproteinases (MMPs) bound to dentin matrices are activated during adhesive bonding procedures and are thought to contribute to the progressive degradation of resin-dentin bonds over time. The purpose of this study was to evaluate the changes in mechanical, biochemical and structural properties of demineralized dentin treated with or without chlorhexidine (CHX), a known MMP-inhibitor. After demineralizing dentin beams in EDTA or phosphoric acid (PA), the baseline modulus of elasticity (E) of each beam was measured by 3-point flexure. Specimens were pretreated with water (control) or with 2% CHX (experimental) and then incubated in artificial saliva (AS) at 37°C for 4 weeks. The E of each specimen was remeasured weekly and, the media was analyzed for solubilized dentin collagen at first and fourth week of incubation. Some specimens were processed for electron microscopy (TEM) immediately after demineralization and after 4 weeks of incubation. In EDTA and PA-demineralized specimens, the E of the control specimens fell (p<0.05) after incubation in AS, while there were no changes in E in the CHX-pretreated specimens over time. More collagen was solubilized from PA-demineralized controls (p<0.05) than from EDTA-demineralized matrices after 1 or 4 weeks. Less collagen (p<0.05) was solubilized from CHX-pretreated specimens demineralized in EDTA compared to PA. TEM examination of control beams revealed that prolonged demineralization of dentin in 10% PA (12 h) did not denature the collagen fibrils.
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影响因子：
2.9
作者：
JAMALL, IS;FINELLI, VN;HEE, SSQ
通讯作者：
HEE, SSQ
影响因子：
3
作者：
Carvalho, RM;Yoshiyama, M;Pashley, DH
通讯作者：
Pashley, DH
影响因子：
7.6
作者：
Pashley, DH;Zhang, Y;Russell, CM
通讯作者：
Russell, CM
DOI：
10.1002/jbm.b.10560
发表时间：
2003-07-15
影响因子：
3.4
作者：
Hashimoto, M;Tay, FR;Oguchi, H
通讯作者：
Oguchi, H
影响因子：
5
作者：
Carrilho, MRD;Tay, FR;Carvalho, RM
通讯作者：
Carvalho, RM