Dephosphorylation of the Ndc80 Tail Stabilizes Kinetochore-Microtubule Attachments via the Ska Complex.
Dephosphorylation of the Ndc80 Tail Stabilizes Kinetochore-Microtubule Attachments via the Ska Complex.
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NDC80尾部的去磷酸化稳定了KineTochore-Microubulule附件，通过SKA络合物。
DOI：
10.1016/j.devcel.2017.04.013
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发表时间：
2017-05-22
影响因子：
11.8
通讯作者：
Desai A
中科院分区：
文献类型：
--
作者：
Cheerambathur DK;Prevo B;Hattersley N;Lewellyn L;Corbett KD;Oegema K;Desai A
在细胞分裂期间，基因组遗传是由有丝分裂染色体的动粒处形成的微管附着物协调的。在着丝粒，Ndc 80复合物的主要微管耦合器，调节极光激酶磷酸化的N-末端尾巴。去磷酸化被提议通过加强尾部与微管晶格的静电相互作用来稳定激动剂-微管附着。在这里，我们表明，去除Ndc 80尾，这妥协在体外微管结合，并没有影响在C。线虫胚胎尽管如此，防止尾部的极光磷酸化导致过早稳定的附着，抑制纺锤体伸长。这种过早的稳定需要保守的微管结合斯卡复合物，它丰富的附着位点之前，后期开始抑制染色体运动。我们建议，Ndc 80尾部去磷酸化促进稳定的kinetochore-microtubule附件通过斯卡复杂的，这种机制，确保准确的分离，通过限制染色体运动后，在纺锤体上的双向取向。极光激酶的磷酸化调节有丝分裂过程中的着丝粒-微管附着。Cheerambathur等人的研究表明，在C.线虫单细胞胚胎中，极光磷酸化的Ndc 80复合物，在着丝粒的主要微管结合因子，控制着丝粒微管附件间接通过调节募集效应，微管结合斯卡复合物。
During cell division, genome inheritance is orchestrated by microtubule attachments formed at kinetochores of mitotic chromosomes. The primary microtubule coupler at the kinetochore, the Ndc80 complex, is regulated by Aurora kinase phosphorylation of its N-terminal tail. Dephosphorylation is proposed to stabilize kinetochore-microtubule attachments by strengthening electrostatic interactions of the tail with the microtubule lattice. Here, we show that removal of the Ndc80 tail, which compromises in vitro microtubule binding, has no effect on kinetochore-microtubule attachments in the C. elegans embryo. Despite this, preventing Aurora phosphorylation of the tail results in prematurely stable attachments that restrain spindle elongation. This premature stabilization requires the conserved microtubule-binding Ska complex, which enriches at attachment sites prior to anaphase onset to dampen chromosome motion. We propose that Ndc80 tail dephosphorylation promotes stabilization of kinetochore-microtubule attachments via the Ska complex and that this mechanism ensures accurate segregation by constraining chromosome motion following biorientation on the spindle. Phosphorylation by Aurora kinases regulates kinetochore-microtubule attachments during mitosis. Cheerambathur et al. show that in C. elegans one-cell embryos, Aurora phosphorylation of the Ndc80 complex, the major microtubule-binding factor at the kinetochore, controls kinetochore-microtubule attachments indirectly by regulating the recruitment of an effector, the microtubule-binding Ska complex.
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DOI：
10.1038/nrm3494
发表时间：
2013-01
期刊：
Nature reviews. Molecular cell biology
影响因子：
--
作者：
通讯作者：
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动力学 - 微管附着依赖于Hec1的无序N末端尾部结构域。
DOI：
10.1016/j.cub.2008.08.012
发表时间：
2008-11-25
期刊：
Current biology : CB
影响因子：
--
作者：
Guimaraes GJ;Dong Y;McEwen BF;Deluca JG
通讯作者：
Deluca JG
影响因子：
64.5
作者：
Miller MP;Asbury CL;Biggins S
通讯作者：
Biggins S
影响因子：
7.5
作者：
Cheerambathur, Dhanya K.;Desai, Arshad
通讯作者：
Desai, Arshad
影响因子：
7.8
作者：
Kim, Taekyung;Moyle, Mark W.;Desai, Arshad
通讯作者：
Desai, Arshad