Altered regulation of cardiac glycogen metabolism in spontaneously diabetic rats.
Altered regulation of cardiac glycogen metabolism in spontaneously diabetic rats.
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改变自发性糖尿病大鼠心肌糖原代谢的调节。
DOI：
10.1152/ajpendo.1983.245.4.e379
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
MillerJr,TB
中科院分区：
文献类型：
--
作者：
MillerJr,TB
对来自对照生物育种/伍斯特（BB/W）大鼠和自发性糖尿病BB/W大鼠的离体灌注心脏进行了研究，以确定是否可以在这种非化学诱导的胰岛素缺乏大鼠中观察到在四氧嘧啶糖尿病大鼠中表达的参与糖原代谢的酶的调节中的代谢异常。用10（-8）M胰岛素灌注对照大鼠心脏10分钟，导致糖原合成酶激活（30%合成酶I不含胰岛素，44%合成酶I含胰岛素）。灌注BB/W糖尿病大鼠的心脏表现出缺乏急性合成酶激活与胰岛素和合成酶磷酸酶活性下降45%。用0.28 μ M肾上腺素灌注BB/W糖尿病大鼠的心脏1分钟，导致磷酸化酶（44%磷酸化酶a）比在相同条件下灌注的BB/W对照心脏（31%磷酸化酶a）中观察到的更大的激活。肾上腺素在BB/W对照组和糖尿病大鼠灌流心脏中产生了类似的环AMP积累、蛋白激酶活化和磷酸化酶激酶活化的变化。此外，磷酸化酶磷酸酶活性没有改变肾上腺素或胰岛素缺乏。这些研究进一步证明了BB/W糖尿病大鼠中的代谢异常，其可归因于非化学诱导的胰岛素依赖性糖尿病模型中的胰岛素缺乏。
Isolated perfused hearts from control Bio-Breeding/Worcester (BB/W) rats and spontaneously diabetic BB/W rats were studied to determine whether metabolic abnormalities that are expressed in alloxan-diabetic rats in the regulation of enzymes involved in glycogen metabolism could be observed in this non-chemically induced insulin-deficient rat. Perfusion of hearts from control rats with 10(-8) M insulin for 10 min resulted in activation of glycogen synthase (30% synthase I without insulin to 44% synthase I with insulin). Perfusion of hearts from BB/W diabetic rats demonstrated a lack of acute synthase activation with insulin and a 45% decrease in synthase phosphatase activity. Perfusion of hearts from BB/W diabetic rats with 0.28 microM epinephrine for 1 min resulted in a greater activation of phosphorylase (44% phosphorylase a) than that observed in BB/W control hearts (31% phosphorylase a) perfused under the same conditions. Epinephrine produced similar changes in cyclic AMP accumulation, protein kinase activation, and phosphorylase kinase activation in perfused hearts of BB/W control and diabetic rats. Further, phosphorylase phosphatase activities were not changed by epinephrine or insulin deficiency. These studies further document metabolic abnormalities in the BB/W diabetic rat that are attributable to insulin deficiency in a non-chemically induced model for insulin-dependent diabetes.
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胰岛素对灌注大鼠心脏糖原合成和合成酶活性的影响。
DOI：
10.1016/0304-4165(72)90249-8
发表时间：
1972
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
S. Adolfsson;O. Isaksson;Å. Hjalmarson
通讯作者：
Å. Hjalmarson
影响因子：
4.8
作者：
H. E. Morgan;M. J. Henderson;Regen Dm;Charles R. Park
通讯作者：
Charles R. Park
环 AMP 依赖性蛋白激酶的测定。
影响因子：
--
作者：
J. Corbin;E. Reimann
通讯作者：
E. Reimann
影响因子：
9.2
作者：
Cunha-Ferreira, Ines;Bento, Ines;Bettencourt-Dias, Monica
通讯作者：
Bettencourt-Dias, Monica
胰岛素对正常和胰岛素缺乏大鼠灌注工作心脏和兰根多夫心脏中葡萄糖代谢模式的影响。
影响因子：
4.1
作者：
E. Chain;K. Mansford;L. Opie
通讯作者：
L. Opie