A Cell/Cilia Cycle Biosensor for Single-Cell Kinetics Reveals Persistence of Cilia after G1/S Transition Is a General Property in Cells and Mice.
A Cell/Cilia Cycle Biosensor for Single-Cell Kinetics Reveals Persistence of Cilia after G1/S Transition Is a General Property in Cells and Mice.
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DOI：
10.1016/j.devcel.2018.10.027
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发表时间：
2018-11-19
影响因子：
11.8
通讯作者：
Mort RL
中科院分区：
文献类型：
--
作者：
Ford MJ;Yeyati PL;Mali GR;Keighren MA;Waddell SH;Mjoseng HK;Douglas AT;Hall EA;Sakaue-Sawano A;Miyawaki A;Meehan RR;Boulter L;Jackson IJ;Mill P;Mort RL
纤毛和细胞周期是密不可分的。纤毛基体中的中心粒使纤毛轴丝成核，并在中心体处隔离中心粒周围基质（PCM）以组织有丝分裂纺锤体。纤毛本身对生长信号作出反应，促使纤毛吸收和细胞周期重新进入。我们描述了一种荧光纤毛和细胞周期生物传感器，允许细胞周期进程和纤毛组装和拆卸动力学的细胞和诱导小鼠的活成像。我们定义组装和拆卸与细胞周期阶段的单细胞分辨率和探索纤毛动力学的细胞间异质性。在分析的所有细胞和组织中，我们观察到纤毛持续通过G1/S过渡并进入S/G2/M期。我们的结论是，持久性的纤毛后，G1/S过渡是一个普遍的属性。该资源将在单个细胞水平上阐明纤毛和细胞周期在发育，再生和疾病中的相互作用。Arl 13 b Cerulean-Fucci 2a生物传感器标记细胞和纤毛周期细胞和小鼠的分析揭示了G1/S转换后纤毛的持久性诱导小鼠系允许谱系追踪和离体活体成像生物体可以耐受人工延长的纤毛而没有明显的表型。纤毛和细胞周期是通过共享机制耦合的基本过程。福特等人开发和表征一种多顺反子生物传感器，可以同时标记小鼠的细胞和纤毛周期，从而能够对其动力学进行实时成像研究。
The cilia and cell cycles are inextricably linked. Centrioles in the basal body of cilia nucleate the ciliary axoneme and sequester pericentriolar matrix (PCM) at the centrosome to organize the mitotic spindle. Cilia themselves respond to growth signals, prompting cilia resorption and cell cycle re-entry. We describe a fluorescent cilia and cell cycle biosensor allowing live imaging of cell cycle progression and cilia assembly and disassembly kinetics in cells and inducible mice. We define assembly and disassembly in relation to cell cycle stage with single-cell resolution and explore the intercellular heterogeneity in cilia kinetics. In all cells and tissues analyzed, we observed cilia that persist through the G1/S transition and into S/G2/M-phase. We conclude that persistence of cilia after the G1/S transition is a general property. This resource will shed light at an individual cell level on the interplay between the cilia and cell cycles in development, regeneration, and disease. Arl13bCerulean-Fucci2a biosensor labels the cell and cilia cycles Analysis of cells and mice reveals persistence of cilia after the G1/S transition Inducible mouse line allows lineage tracing and ex vivo live imaging Organisms can tolerate artificially lengthened cilia without overt phenotypes. The cilia and cell cycles are fundamental processes coupled through shared machinery. Ford et al. develop and characterize a multicistronic biosensor that can simultaneously label the cell and cilia cycles in mice, enabling live imaging studies of their kinetics.
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影响因子：
3.7
作者：
Hassounah NB;Nagle R;Saboda K;Roe DJ;Dalkin BL;McDermott KM
通讯作者：
McDermott KM
影响因子：
64.8
作者：
通讯作者：
--
影响因子：
16.2
作者：
Lehtinen MK;Zappaterra MW;Chen X;Yang YJ;Hill AD;Lun M;Maynard T;Gonzalez D;Kim S;Ye P;D'Ercole AJ;Wong ET;LaMantia AS;Walsh CA
通讯作者：
Walsh CA
影响因子：
1.5
作者：
Engert, Silvia;Liao, W. Perry;Lickert, Heiko
通讯作者：
Lickert, Heiko
影响因子：
21.3
作者：
Borovina, Antonia;Superina, Simone;Ciruna, Brian
通讯作者：
Ciruna, Brian