A universal trend of reduced mRNA stability near the translation-initiation site in prokaryotes and eukaryotes.
A universal trend of reduced mRNA stability near the translation-initiation site in prokaryotes and eukaryotes.
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原核生物和真核生物翻译起始位点附近 mRNA 稳定性降低的普遍趋势
DOI：
10.1371/journal.pcbi.1000664
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发表时间：
2010-02-05
影响因子：
4.3
通讯作者：
Wilke CO
中科院分区：
文献类型：
--
作者：
Gu W;Zhou T;Wilke CO
最近的研究表明，起始密码子附近的mRNA二级结构的热力学稳定性可以调节大肠杆菌中的翻译效率，二级结构越不稳定，翻译效率越高。我们调查了 340 个物种的完整基因组，寻找起始密码子附近 mRNA 二级结构减少的信号。我们的分析包括细菌、古细菌、真菌、植物、昆虫、鱼类、鸟类和哺乳动物。我们发现几乎所有物种都显示出起始密码子附近 mRNA 稳定性降低的证据。稳定性的降低通常随着基因组 GC 含量的增加而增加。在原核生物中，这种减少也随着最佳生长温度的降低而增加。在基因组内，基因之间的稳定性存在差异，这种差异与基因GC含量、密码子偏倚和基因表达水平相关。然而，对于鸟类和哺乳动物，我们没有发现起始密码子附近 mRNA 稳定性降低的全基因组趋势。然而，这些生物体中最富含 GC 的基因确实显示出这样的信号。我们得出结论，起始密码子附近的 mRNA 二级结构稳定性降低是所有细胞生命的普遍特征。我们认为这种减少的根源是选择起始密码子被起始tRNA有效识别。
Recent studies have suggested that the thermodynamic stability of mRNA secondary structure near the start codon can regulate translation efficiency in Escherichia coli, and that translation is more efficient the less stable the secondary structure. We survey the complete genomes of 340 species for signals of reduced mRNA secondary structure near the start codon. Our analysis includes bacteria, archaea, fungi, plants, insects, fishes, birds, and mammals. We find that nearly all species show evidence for reduced mRNA stability near the start codon. The reduction in stability generally increases with increasing genomic GC content. In prokaryotes, the reduction also increases with decreasing optimal growth temperature. Within genomes, there is variation in the stability among genes, and this variation correlates with gene GC content, codon bias, and gene expression level. For birds and mammals, however, we do not find a genome-wide trend of reduced mRNA stability near the start codon. Yet the most GC rich genes in these organisms do show such a signal. We conclude that reduced stability of the mRNA secondary structure near the start codon is a universal feature of all cellular life. We suggest that the origin of this reduction is selection for efficient recognition of the start codon by initiator-tRNA.
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影响因子：
4.5
作者：
Hoede C;Denamur E;Tenaillon O
通讯作者：
Tenaillon O
影响因子：
1.8
作者：
HOFACKER, IL;FONTANA, W;SCHUSTER, P
通讯作者：
SCHUSTER, P
影响因子：
5.7
作者：
Eames, Matt;Kortemme, Tanja
通讯作者：
Kortemme, Tanja
DOI：
10.1016/s0006-291x(02)00226-7
发表时间：
2002-04-26
影响因子：
3.1
作者：
Cortazzo, P;Cerve単ansky, C;Deana, A
通讯作者：
Deana, A
影响因子：
10.7
作者：
Drummond, DA;Raval, A;Wilke, CO
通讯作者：
Wilke, CO