Real-Time Multi-Angle Projection Imaging of Biological Dynamics
Real-Time Multi-Angle Projection Imaging of Biological Dynamics
复制标题
生物动力学实时多角度投影成像
DOI：
10.1101/2020.10.29.355917
复制
发表时间：
2020
期刊：
影响因子：
--
通讯作者：
R. Fiolka
中科院分区：
文献类型：
--
作者：
Bo;Etai Sapoznik;Theresa Pohlkamp;Tamara S. Terrones;Erik S. Welf;James D. Manton;Philippe Roudot;K. Hake;L. Whitehead;Andrew G. York;K. Dean;R. Fiolka
我们推出了一种具有成本效益且易于实施的扫描单元，使任何基于相机的显微镜都能从不同的视角进行投影成像。我们通过快速提供具有不妥协的横向分辨率和高光学对比度的投影图像，在点阵光片和斜平面显微镜上展示了这种能力。通过从一个或多个视角对样品进行成像，我们的方法能够从仅两个图像可视化快速生物过程、真实的时间立体成像以及整个细胞体积的三维颗粒定位。此外，由于我们的投影成像技术提供了直观的实时三维渲染，它提高了显微镜的可用性，使用户能够更容易地优化仪器性能，并在运行中识别感兴趣的生物现象，同时还将数据开销减少了100倍以上。我们利用我们的快速投影方法来成像培养的神经元中的癌细胞形态动力学和钙信号传导，对遗传编码的纳米颗粒进行三维定位，以及同时成像胚胎斑马鱼心脏的正交视图。
We introduce a cost-effective and easily implemented scan unit which enables any camera-based microscope to perform projection imaging from diverse viewing angles. We demonstrate this capability on Lattice Light-Sheet and Oblique Plane Microscopy by rapidly delivering projection images with an uncompromised lateral resolution and high optical contrast. By imaging the sample from one or multiple perspectives, our method enables visualization of rapid biological processes, real time stereoscopic imaging as well as three-dimensional particle localization throughout a cellular volume from just two images. Furthermore, because our projection imaging technique provides intuitive three-dimensional renderings in real-time, it improves microscope usability, allows users to more-readily optimize instrument performance and identify biological phenomena of interest on-the-fly, while also reducing data overhead by a factor of >100. We leverage our rapid projection method to image cancer cell morpho-dynamics and calcium signaling in cultured neurons, to perform three-dimensional localization of genetically encoded nanoparticles, as well as to image orthogonal views of an embryonic Zebrafish heart simultaneously.
影响因子：
10.4
作者：
Dean KM;Roudot P;Welf ES;Pohlkamp T;Garrelts G;Herz J;Fiolka R
通讯作者：
Fiolka R
影响因子：
48
作者：
Mickoleit, Michaela;Schmid, Benjamin;Huisken, Jan
通讯作者：
Huisken, Jan