A colorimetric biosensor based on guanidinium recognition for the assay of protein tyrosine phosphatase 1B and its inhibitors
A colorimetric biosensor based on guanidinium recognition for the assay of protein tyrosine phosphatase 1B and its inhibitors
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基于胍识别的比色生物传感器用于检测蛋白酪氨酸磷酸酶1B及其抑制剂
DOI：
10.1039/c7nj02918g
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发表时间：
2017-11
影响因子：
3.3
通讯作者：
Zhang Juan
中科院分区：
文献类型：
--
作者：
Lv Jun;Chen Tingjun;Yue Xiquan;Zhou Jianqiong;Gong Xiuqing;Zhang Juan
基于精氨酸-甘氨酸-半胱氨酸（RGC）修饰的金纳米粒子（RGC/AuNPs）与4-氨基苯基磷酸功能化的Fe 3 O 4磁性纳米粒子（MNP/APP）的胍识别辅助配位作用，建立了一种检测蛋白酪氨酸磷酸酶1B（PTP 1B）及其抑制剂的比色生物传感器。PTP 1B可催化磷酸酯键的断裂和磷酸基团从MNP/APP表面的脱离，导致相应的配位反应性的丧失。在加入RGC/AuNP的溶液后，RGC/AuNP在磁分离后保留在上清液中，并且观察到高吸光度值。因此，一个简单的比色传感器PTP 1B的检测。在优化的实验条件下，PTP 1B的检测线性范围为0.002 U mL−1至0.08 U mL−1，最低检测限为0.0013 U mL−1。此外，利用该生物传感器测定了桦木酸和6-氯-3-甲酰基-7-甲基色酮对PTP 1B的抑制作用，IC 50值分别为9 μM和15 μM。因此，这种新的生物传感器不仅具有很大的潜力，PTP 1B的分析，而且其抑制剂的检测。
In this study, a colorimetric biosensor was established for the detection of protein tyrosine phosphatase 1B (PTP1B) and its inhibitor based on the guanidinium recognition assisted coordination of arginine-glycine-cysteine (RGC) modified gold nanoparticles (RGC/AuNPs) with 4-aminophenyl phosphate-functionalized Fe3O4 magnetic nanoparticles (MNPs/APP). PTP1B can catalyze the cleavage of the phosphate ester bond and the departure of the phosphate acid group from the surface of MNPs/APP, resulting in the loss of the corresponding coordination reactivity. Upon the addition of a solution of RGC/AuNPs, RGC/AuNPs remained in the supernatant solution after magnetic separation and a high absorbance value was observed. Thus, a simple colorimetric biosensor for PTP1B assay was developed. Under the optimized experimental conditions, PTP1B was detected within a linear range of 0.002 U mL−1 to 0.08 U mL−1 with the lowest detection limit of 0.0013 U mL−1. Moreover, using this proposed biosensor, the inhibition effect of betulinic acid and 6-chloro-3-formyl-7-methylchromone on PTP1B is determined with IC50 values of 9 μM and 15 μM, respectively. Therefore, this new biosensor not only has great potential for PTP1B analysis but also for the detection of its inhibitors.
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影响因子：
2.7
作者：
Yi Sup Shim;Ki Chul Kim;D. Chi;Keun-Hyeung Lee;Hyeongjin Cho
通讯作者：
Yi Sup Shim;Ki Chul Kim;D. Chi;Keun-Hyeung Lee;Hyeongjin Cho
影响因子：
15
作者：
Xiao, Qing;Luechapanichkul, Rinrada;Zhai, Yujing;Pei, Dehua
通讯作者：
Pei, Dehua
制造磁控可移动结构以开发可重复使用的电化学生物传感器
影响因子：
4.6
作者：
Zhu X;Feng C;Ye Z;Chen Y;Li G
通讯作者：
Li G
影响因子：
37.8
作者：
Lanahan AA;Lech D;Dubrac A;Zhang J;Zhuang ZW;Eichmann A;Simons M
通讯作者：
Simons M
影响因子：
1.2
作者：
Wei Zou
通讯作者：
Wei Zou