A molecular atlas reveals the tri-sectional spinning mechanism of spider dragline silk.
A molecular atlas reveals the tri-sectional spinning mechanism of spider dragline silk.
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DOI：
10.1038/s41467-023-36545-6
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发表时间：
2023-02-15
影响因子：
16.6
通讯作者：
Wang, Yi
中科院分区：
文献类型：
--
作者：
Hu, Wenbo;Jia, Anqiang;Ma, Sanyuan;Zhang, Guoqing;Wei, Zhaoyuan;Lu, Fang;Luo, Yongjiang;Zhang, Zhisheng;Sun, Jiahe;Yang, Tianfang;Xia, TingTing;Li, Qinhui;Yao, Ting;Zheng, Jiangyu;Jiang, Zijie;Xu, Zehui;Xia, Qingyou;Wang, Yi
蜘蛛的大壶腹腺（Ma）中产生天然丝的过程赋予拖丝以非凡的机械性能和仿生应用的潜力。然而，Ma腺在这一过程中的确切遗传作用仍然未知。在这里，我们进行了一个系统的拖丝生产的分子图谱，通过一个高质量的基因组组装的金球编织蜘蛛Trichonephila clavata和多组学的方法来定义马腺三节结构：尾巴，囊，导管。我们发现了一个层次的生物合成的蛛丝蛋白，有机酸，脂质，几丁质在部分马腺致力于罚款丝宪法。蛛丝蛋白的有序分泌是通过表观遗传和ceRNA标签对基因组群分布的蛛丝蛋白基因的协同调节实现的。单细胞和空间RNA分析确定了10种细胞类型与分区的功能划分确定三节组织的马腺。收敛分析和遗传操作进一步证实，这种三节结构的丝腺是类似的节肢动物和丝的形成密不可分。总的来说，我们的研究提供了多维数据，大大扩展了蜘蛛拖丝生成的知识，并最终有利于蜘蛛灵感纤维的创新。蜘蛛大壶状腺（Ma）产丝的遗传基础仍然未知。Hu等人结合基因组组装和多组学，揭示了金球织蜘蛛该腺体的分子图谱，揭示了Ma腺体中产丝的单细胞空间结构。
The process of natural silk production in the spider major ampullate (Ma) gland endows dragline silk with extraordinary mechanical properties and the potential for biomimetic applications. However, the precise genetic roles of the Ma gland during this process remain unknown. Here, we performed a systematic molecular atlas of dragline silk production through a high-quality genome assembly for the golden orb-weaving spider Trichonephila clavata and a multiomics approach to defining the Ma gland tri-sectional architecture: Tail, Sac, and Duct. We uncovered a hierarchical biosynthesis of spidroins, organic acids, lipids, and chitin in the sectionalized Ma gland dedicated to fine silk constitution. The ordered secretion of spidroins was achieved by the synergetic regulation of epigenetic and ceRNA signatures for genomic group-distributed spidroin genes. Single-cellular and spatial RNA profiling identified ten cell types with partitioned functional division determining the tri-sectional organization of the Ma gland. Convergence analysis and genetic manipulation further validated that this tri-sectional architecture of the silk gland was analogous across Arthropoda and inextricably linked with silk formation. Collectively, our study provides multidimensional data that significantly expand the knowledge of spider dragline silk generation and ultimately benefit innovation in spider-inspired fibers. The genetic basis of spider major ampullate (Ma) gland silk production remains unknown. Hu et al. unveil a molecular atlas of this gland for the golden orb-weaving spider combining genome assembly and multiomics, revealing the single-cell spatial architecture of silk production in the Ma gland.
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影响因子：
2.7
作者：
Garrison NL;Rodriguez J;Agnarsson I;Coddington JA;Griswold CE;Hamilton CA;Hedin M;Kocot KM;Ledford JM;Bond JE
通讯作者：
Bond JE
影响因子：
14.9
作者：
Chen T;Ma J;Liu Y;Chen Z;Xiao N;Lu Y;Fu Y;Yang C;Li M;Wu S;Wang X;Li D;He F;Hermjakob H;Zhu Y
通讯作者：
Zhu Y
影响因子：
64.8
作者：
Jin, HJ;Kaplan, DL
通讯作者：
Kaplan, DL
影响因子：
6.2
作者：
Andersson, Marlene;Holm, Lena;Rising, Anna
通讯作者：
Rising, Anna
影响因子：
9.2
作者：
Fan Z;Yuan T;Liu P;Wang LY;Jin JF;Zhang F;Zhang ZS
通讯作者：
Zhang ZS