Beyond Saccharomyces pastorianus for modern lager brews: Exploring non-cerevisiae Saccharomyces hybrids with heterotic maltotriose consumption and novel aroma profile.
Beyond Saccharomyces pastorianus for modern lager brews: Exploring non-cerevisiae Saccharomyces hybrids with heterotic maltotriose consumption and novel aroma profile.
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DOI：
10.3389/fmicb.2022.1025132
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发表时间：
2022
影响因子：
5.2
通讯作者：
Louis, Edward J.
中科院分区：
文献类型：
--
作者：
Gyurchev, Nikola Y.;Coral-Medina, Angela;Weening, Susan M.;Almayouf, Salwa;Kuijpers, Niels G. A.;Nevoigt, Elke;Louis, Edward J.
关键词：
非驯化的野生酵母菌对啤酒多样化具有很好的特性，特别是当用于从头产生种间杂交种时。目前工作的一个主要动机是问题是否有吸引力的新的酵母种间杂种可以创建用于生产异国情调的拉格啤酒，而不使用的基因组资源的啤酒酵母酿酒酵母。重要的是，麦芽三糖利用是通常与驯化的爱尔啤酒/贮藏啤酒酿造菌株相关的基本特征。对代表酵母属所有8个种的近200株菌株进行了高通量筛选。三个酿酒酵母菌株能够有氧生长麦芽三糖作为唯一的碳源，一个性状，直到最近未确定的这个物种。我们的筛选也证实了最近报道的S. jurei菌株D5095 T。值得注意的是，利用麦芽三糖的S。mikalgae或S. jurei菌株和麦芽三糖阴性的Saccharomyces eubayanus菌株CBS 12357 T显示出杂种优势，并且在有氧利用麦芽三糖作为唯一碳源方面优于两个亲本。事实上，这种糖的最大比生长速率与众所周知的工业菌株巴斯德酵母CBS 1513相当。在啤酒酿造环境（氧气有限），新的杂交能够发酵麦芽糖，而麦芽三糖没有代谢。有利的果味酯的产生，表明新的杂交种有可能增加啤酒酿造的多样性。
Non-domesticated, wild Saccharomyces yeasts have promising characteristics for beer diversification, particularly when used in the generation of de novo interspecific hybrids. A major motivation for the current work was the question whether attractive novel Saccharomyces interspecific hybrids can be created for the production of exotic lager beers without using the genomic resources of the ale yeast Saccharomyces cerevisiae. Importantly, maltotriose utilization is an essential characteristic typically associated with domesticated ale/lager brewing strains. A high-throughput screening on nearly 200 strains representing all eight species of the Saccharomyces genus was conducted. Three Saccharomyces mikatae strains were able to aerobically grow on maltotriose as the sole carbon source, a trait until recently unidentified for this species. Our screening also confirmed the recently reported maltotriose utilization of the S. jurei strain D5095T. Remarkably, de novo hybrids between a maltotriose-utilizing S. mikatae or S. jurei strain and the maltotriose-negative Saccharomyces eubayanus strain CBS 12357T displayed heterosis and outperformed both parents with regard to aerobically utilizing maltotriose as the sole source of carbon. Indeed, the maximum specific growth rates on this sugar were comparable to the well-known industrial strain, Saccharomyces pastorianus CBS 1513. In lager brewing settings (oxygen-limited), the new hybrids were able to ferment maltose, while maltotriose was not metabolized. Favorable fruity esters were produced, demonstrating that the novel hybrids have the potential to add to the diversity of lager brewing.
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影响因子：
4.4
作者：
Brouwers, Nick;Brickwedde, Anja;Daran, Jean-Marc G.
通讯作者：
Daran, Jean-Marc G.
现象：用于固体培养基上微生物生长表型的高通量和灵活工具。
影响因子：
4.2
作者：
Barton DBH;Georghiou D;Dave N;Alghamdi M;Walsh TA;Louis EJ;Foster SS
通讯作者：
Foster SS
影响因子：
5.1
作者：
Eizaguirre, Juan I.;Peris, David;Libkind, Diego
通讯作者：
Libkind, Diego
影响因子：
2.6
作者：
Dietvorst, J;Londesborough, J;Steensma, HY
通讯作者：
Steensma, HY
影响因子：
4.5
作者：
Baker, EmilyClare P.;Hittinger, Chris Todd
通讯作者：
Hittinger, Chris Todd