Fast Recognition of Lecanicillium spp., and Its Virulence Against Frankliniella occidentalis.
Fast Recognition of Lecanicillium spp., and Its Virulence Against Frankliniella occidentalis.
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DOI：
10.3389/fmicb.2020.561381
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发表时间：
2020
影响因子：
5.2
通讯作者：
Jiang T
中科院分区：
文献类型：
--
作者：
Zhou Y;Zou X;Zhi J;Xie J;Jiang T
西氏富兰氏菌(Frankliniella Ocicientalis)是一种高度吸食作物的害虫。昆虫病原真菌Lecanicillium spp.是重要的害虫病原菌，一些品种已被开发为商品化的生物农药。对链球菌属进行了探索。为了开发更有效的西瓜疫霉菌对照，从内部转录间隔区(ITS)、SSU、LSU、TEF、RPB1和RPB2基因中筛选出用于菌株鉴定的高效条形码组合。利用6个基因构建了链球菌属的系统发育关系。结果表明，ITS、TEF、RPB1和RPB2可用于菌株的鉴定。由这四个基因自由组合构建的所有系统发育树都显示出几乎相同的拓扑结构。对一种纯化的分生孢子悬浮液的生物活性测定进一步证实了7株Lecanicillium菌株对2龄若虫和成虫的侵染。弱毒乳杆菌菌株GZUIFR-LUN1405的毒力最强，暴露7d后对西方小食心虫成虫的致死率约为91.67%，对若虫的致死率约为76.67%。选用衰弱乳杆菌菌株GZUIFR-LUN1405和球茎乳杆菌菌株GZUIFR-ZHJ01，比较两种真菌对西方拟青霉产卵量的影响。GZUIFR-ZHJ01菌株处理成虫后，其若虫数量明显减少。ITS和RPB1的结合可用于链球菌属的快速识别。本文首次报道了衰弱乳杆菌、球茎乳杆菌、产蛛L乳杆菌和aphanocladii乳杆菌对西葫芦的致病力。另外，根结线虫菌株GZUIFR-ZHJ01对西方根结线虫有较高的致死率，并能抑制其繁殖力。
Frankliniella occidentalis (Thysanoptera: Thripidae) is a highly rasping-sucking pest of numerous crops. The entomogenous fungi of Lecanicillium spp. are important pathogens of insect pests, and some species have been developed as commercial biopesticides. To explore Lecanicillium spp. resources in the development of more effective F. occidentalis controls, efficient barcode combinations for strain identification were screened from internal transcribed spacers (ITS), SSU, LSU, TEF, RPB1, and RPB2 genes. Six genes were used to reconstruct Lecanicillium genus phylogeny. The results showed that ITS, TEF, RPB1, and RPB2 could be used to identify the strains. All phylogenetic trees reconstructed by free combination of these four genes exhibited almost the same topology. Bioassay studies of a purified conidial suspension further confirmed the infection of second-instar nymphs and adult female F. occidentalis by seven Lecanicillium strains. L. attenuatum strain GZUIFR-lun1405 was the most virulent, killing approximately 91.67% F. occidentalis adults and 76.67% nymphs after a 7-day exposure. L. attenuatum strain GZUIFR-lun1405 and L. cauligalbarum strain GZUIFR-ZHJ01 were selected to compare the fungal effects on the number of eggs laid by F. occidentalis. The number of F. occidentalis nymphs significantly decreased when F. occidentalis adults were treated with L. cauligalbarum strain GZUIFR-ZHJ01. The combination of ITS and RPB1 could be used for fast recognition of Lecanicillium spp. This is the first report of the pathogenicity of L. attenuatum, L. cauligalbarum, L araneogenum, and L. aphanocladii against F. occidentalis. Additionally, L. cauligalbarum strain GZUIFR-ZHJ01 caused high F. occidentalis mortality and inhibited the fecundity of the pest.
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影响因子：
1.1
作者：
Chen, Wan-Hao;Han, Yan-Feng;Jin, Dao-Chao
通讯作者：
Jin, Dao-Chao
影响因子：
2.5
作者：
Lee, Se Jin;Kim, Sihyeon;Kim, Jae Su
通讯作者：
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影响因子：
1.9
作者：
Loughner, RL;Warnock, DF;Cloyd, RA
通讯作者：
Cloyd, RA
影响因子：
1.4
作者：
Gao, Yulin;Reitz, Stuart R.;Lei, Zhongren
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影响因子：
4.1
作者：
Mouden S;Sarmiento KF;Klinkhamer PG;Leiss KA
通讯作者：
Leiss KA