Comparison of pelvic muscle architecture between humans and commonly used laboratory species.
Comparison of pelvic muscle architecture between humans and commonly used laboratory species.
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DOI：
10.1007/s00192-014-2423-9
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发表时间：
2014-11
影响因子：
1.8
通讯作者：
Lieber, Richard L.
中科院分区：
文献类型：
--
作者：
Alperin, Marianna;Tuttle, Lori J.;Conner, Blair R.;Dixon, Danielle M.;Mathewson, Margie A.;Ward, Samuel R.;Lieber, Richard L.
阴道分娩会对盆底肌肉 (PFM) 产生有害影响，从而导致盆底疾病的发生。为了从机制上联系这些事件，需要使用动物模型进行实验，因为获取人类 PFM 组织具有挑战性。在选择动物模型时，有必要对 PFM 设计进行比较研究，因为仅凭大体解剖学不足以指导选择。使用微机械解剖测量人体 PFM 结构，然后使用结构差异指数 (ADI) 与小鼠 (n=10)、大鼠 (n=10) 和兔子 (n=10) 进行比较（参数化肌节长度与最佳肌节比率、纤维与肌肉长度比率以及每块肌肉贡献的 PFM 总质量和生理横截面积 (PCSA) 的综合测量值）。收获尾骨肌 (C)、髂尾肌 (IC) 和耻尾肌 (PC) 并进行结构测量。使用重复测量方差分析 (ANOVA) 和事后 Tukey 检验对物种内的参数进行比较。使用 log-10 转换变量的最小二乘回归来量化跨物种 PFM 的尺度关系。根据 ADI，发现大鼠与人类最相似（ADI = 2.5），其次是小鼠（ADI = 3.3）。当动物的体重相对于肌肉质量、肌肉长度、纤维长度和 PCSA 缩放系数进行回归时，显示出负异速生长关系或比几何缩放预测的增长更小。就常用实验动物的肌肉设计而言，大鼠最接近人类 PFM，其次是小鼠。 PFM 结构参数的负异速生长可能是由于这些肌肉的多方面功能所致。
Pelvic floor muscles (PFM) are deleteriously affected by vaginal birth, which contributes to the development of pelvic floor disorders. To mechanistically link these events, experiments using animal models are required, as access to human PFM tissue is challenging. In choosing an animal model, a comparative study of PFM design is necessary, since gross anatomy alone is insufficient to guide the selection. Human PFM architecture was measured using micromechanical dissection and then compared with mouse (n=10), rat (n=10), and rabbit (n=10) using the Architectural Difference Index (ADI) (parameterizing a combined measure of sarcomere length-to-optimal-sarcomere ratio, fiber-to-muscle-length ratio, and fraction of total PFM mass and physiological cross-sectional area (PCSA) contributed by each muscle). Coccygeus (C), iliocaudalis (IC), and pubocaudalis (PC) were harvested and subjected to architectural measurements. Parameters within species were compared using repeated measures analysis of variance (ANOVA) with post hoc Tukey's tests. The scaling relationships of PFM across species were quantified using least-squares regression of log-10-transformed variables. Based on the ADI, rat was found to be the most similar to humans (ADI = 2.5), followed by mouse (ADI = 3.3). When animals' body mass was regressed against muscle mass, muscle length, fiber length, and PCSA scaling coefficients showed a negative allometric relationship or smaller increase than predicted by geometric scaling. In terms of muscle design among commonly used laboratory animals, rat best approximates the human PFM, followed by mouse. Negative allometric scaling of PFM architectural parameters is likely due to the multifaceted function of these muscles.
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影响因子：
14
作者：
Feinberg, Adam W.;Alford, Patrick W.;Jin, Hongwei;Ripplinger, Crystal M.;Werdich, Andreas A.;Sheehy, Sean P.;Grosberg, Anna;Parker, Kevin Kit
通讯作者：
Parker, Kevin Kit
影响因子：
2.4
作者：
Ward, SR;Lieber, RL
通讯作者：
Lieber, RL
影响因子：
2
作者：
ALEXANDER, RM;JAYES, AS;WATHUTA, EM
通讯作者：
WATHUTA, EM
影响因子：
1.9
作者：
Lieber, Richard L.
通讯作者：
Lieber, Richard L.
影响因子：
3.3
作者：
Kawakami, Y;Ichinose, Y;Fukunaga, T
通讯作者：
Fukunaga, T