Identification and Characterization of the Very-Low-Density Lipoprotein Receptor Gene from Branchiostoma belcheri: Insights into the Origin and Evolution of the Low-Density Lipoprotein Receptor Gene Family.
Identification and Characterization of the Very-Low-Density Lipoprotein Receptor Gene from Branchiostoma belcheri: Insights into the Origin and Evolution of the Low-Density Lipoprotein Receptor Gene Family.
复制标题
贝尔切里群瘤的非常低密度脂蛋白受体基因的鉴定和表征：对低密度脂蛋白受体基因家族的起源和进化的见解。
DOI：
10.3390/ani13132193
复制
发表时间：
2023-07-04
期刊：
影响因子：
--
通讯作者：
中科院分区：
文献类型：
--
作者：
低密度脂蛋白受体（LDLR）家族在脂质代谢中起着至关重要的作用，但迄今为止，该家族尚未在古脊索动物（原脊索动物）中进行研究。我们确定了一个新的低密度脂蛋白受体家族成员的原脊索动物Branchiostoma belcheri。此外，我们还研究了该基因家族的进化过程和分子功能。我们的工作填补了原脊索动物LDLR家族的研究空白，并为脊索动物LDLR的起源和进化提供了新的见解。低密度脂蛋白受体（LDLRs）是一类主要参与胆固醇稳态和细胞信号转导的细胞表面内吞作用受体。极低密度脂蛋白受体（VLDLR）是LDLR家族的成员，被认为是具有多种生理功能的多功能受体。然而，迄今为止，在原索动物中还没有发现VLDLR基因。作为原脊索动物的代表物种，文昌鱼是脊椎动物祖先的最佳范例。文昌鱼中VLDLR基因的鉴定和鉴定对于探讨LDLR家族的进化过程具有重要意义。本研究利用RACE-PCR技术克隆了文昌鱼VLDLR基因（AmphiVLDLR）。AmphiVLDLR基因转录本全长3217 nt，开放阅读框长度为2577 nt，编码858个氨基酸，在脊椎动物VLDLR中高度保守，其一级结构和三维结构均包含5个特征结构域。与其他脊椎动物VLDLR具有19个外显子和18个内含子的保守基因组结构组织不同，AmphiVLDLR具有13个外显子和12个内含子。选择压力分析结果表明，AmphiVLDLR有许多正选择位点。此外，使用RT-qPCR的AmphiVLDLR的组织表达显示，AmphiVLDLR RNA表达水平在鳃和肌肉中最高，在肝盲肠和性腺中中等，在肠中最低。进化分析结果表明，AmphiVLDLR基因是VLDLR家族的一个新成员，其家族成员在进化过程中经历了重复和缺失。这些结果意味着LDLR家族成员的功能也经历了分化。综上所述，我们在文昌鱼中发现了一个新的VLDLR基因同源物（AmphiVLDLR）。我们的研究结果提供了深入了解LDLR基因家族的功能和进化。
The low-density lipoprotein receptor (LDLR) family plays crucial roles in lipid metabolism, but this family has not been investigated in ancient chordates (protochordates) to date. We identified and characterized a new LDLR family member in a protochordate—Branchiostoma belcheri. Additionally, we investigated the evolutionary process and molecular function in this gene family. Our work fills a research gap in the LDLR family in protochordates and provides new insights into the origin and evolution of chordate LDLRs. Low-density lipoprotein receptors (LDLRs) are a class of cell-surface endocytosis receptors that are mainly involved in cholesterol homeostasis and cellular signal transduction. Very-low-density lipoprotein receptors (VLDLRs), which are members of the LDLR family, have been regarded as multi-function receptors that fulfill diverse physiological functions. However, no VLDLR gene has been identified in protochordates to date. As a representative protochordate species, amphioxi are the best available example of vertebrate ancestors. Identifying and characterizing the VLDLR gene in amphioxi has high importance for exploring the evolutionary process of the LDLR family. With this study, a new amphioxus VLDLR gene (designated AmphiVLDLR) was cloned and characterized using RACE-PCR. The 3217 nt transcript of the AmphiVLDLR had a 2577 nt ORF, and the deduced 858 amino acids were highly conserved within vertebrate VLDLRs according to their primary structure and three-dimensional structure, both of which contained five characteristic domains. In contrast to other vertebrate VLDLRs, which had a conserved genomic structure organization with 19 exons and 18 introns, the AmphiVLDLR had 13 exons and 12 introns. The results of a selective pressure analysis showed that the AmphiVLDLR had numerous positive selection sites. Furthermore, the tissue expression of AmphiVLDLR using RT-qPCR showed that AmphiVLDLR RNA expression levels were highest in the gills and muscles, moderate in the hepatic cecum and gonads, and lowest in the intestines. The results of the evolutionary analysis demonstrated that the AmphiVLDLR gene is a new member of the VLDLR family whose family members have experienced duplications and deletions over the evolutionary process. These results imply that the functions of LDLR family members have also undergone differentiation. In summary, we found a new VLDLR gene homolog (AmphiVLDLR) in amphioxi. Our results provide insight into the function and evolution of the LDLR gene family.
登录
查看更多内容
影响因子：
64.8
作者：
Jumper J;Evans R;Pritzel A;Green T;Figurnov M;Ronneberger O;Tunyasuvunakool K;Bates R;Žídek A;Potapenko A;Bridgland A;Meyer C;Kohl SAA;Ballard AJ;Cowie A;Romera-Paredes B;Nikolov S;Jain R;Adler J;Back T;Petersen S;Reiman D;Clancy E;Zielinski M;Steinegger M;Pacholska M;Berghammer T;Bodenstein S;Silver D;Vinyals O;Senior AW;Kavukcuoglu K;Kohli P;Hassabis D
通讯作者：
Hassabis D
影响因子：
15.9
作者：
Herz, J;Strickland, DK
通讯作者：
Strickland, DK
影响因子：
5.5
作者：
Kapustin Y;Souvorov A;Tatusova T;Lipman D
通讯作者：
Lipman D
影响因子：
11.4
作者：
BUJO, H;HERMANN, M;SCHNEIDER, WJ
通讯作者：
SCHNEIDER, WJ
影响因子：
3
作者：
Knapp, Bernhard;Lederer, Nadja;Schreiner, Wolfgang
通讯作者：
Schreiner, Wolfgang