Characterization of host microRNAs that respond to DNA virus infection in a crustacean.
Characterization of host microRNAs that respond to DNA virus infection in a crustacean.
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甲壳动物中响应 DNA 病毒感染的宿主 microRNA 的表征
DOI：
10.1186/1471-2164-13-159
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发表时间：
2012-04-30
期刊：
影响因子：
4.4
通讯作者：
Zhang X
中科院分区：
文献类型：
--
作者：
Huang T;Xu D;Zhang X
背景MicroRNAs（miRNAs）是基因表达的关键转录后调节因子，参与真核细胞的许多过程。已知宿主miRNA的表达谱可以被病毒重塑。然而，海洋无脊椎动物的miRNA，响应病毒infection.ResultsIn这项研究中，虾Marsupenaeus japonicus的系统调查尚未performed. ResultsIn这项研究中，挑战白色斑点综合征病毒（WSSV）。在感染WSSV后不同时间（0、6、24和48 h）对感染WSSV的对虾进行小RNA测序，鉴定出63个宿主miRNAs，其中48个在其他动物中是保守的，代表43个不同的家族。在鉴定的宿主miRNAs中，31个在病毒感染后差异表达，其中25个上调，6个下调。通过北方印迹证实了结果。TargetScan和米兰达算法显示，差异表达的miRNAs中大部分靶基因与免疫应答相关。基因本体论分析表明，这些miRNAs介导了免疫信号通路。进化分析表明，其中三个，miR-1，miR-7和miR-34，是高度保守的虾，果蝇和人类和功能在相似的pathways.ConclusionsOur study provides the first large scale characterization of marine invertebrate miRNAs that respond to virus infection.这将有助于揭示参与由miRNAs介导的病毒-宿主相互作用的分子事件及其在动物中的进化。
BackgroundMicroRNAs (miRNAs) are key posttranscriptional regulators of gene expression that are implicated in many processes of eukaryotic cells. It is known that the expression profiles of host miRNAs can be reshaped by viruses. However, a systematic investigation of marine invertebrate miRNAs that respond to virus infection has not yet been performed.ResultsIn this study, the shrimpMarsupenaeus japonicuswas challenged by white spot syndrome virus (WSSV). Small RNA sequencing of WSSV-infected shrimp at different time post-infection (0, 6, 24 and 48 h) identified 63 host miRNAs, 48 of which were conserved in other animals, representing 43 distinct families. Of the identified host miRNAs, 31 were differentially expressed in response to virus infection, of which 25 were up-regulated and six down-regulated. The results were confirmed by northern blots. The TargetScan and miRanda algorithms showed that most target genes of the differentially expressed miRNAs were related to immune responses. Gene ontology analysis revealed that immune signaling pathways were mediated by these miRNAs. Evolutionary analysis showed that three of them, miR-1, miR-7 and miR-34, are highly conserved in shrimp, fruit fly and humans and function in the similar pathways.ConclusionsOur study provides the first large-scale characterization of marine invertebrate miRNAs that respond to virus infection. This will help to reveal the molecular events involved in virus-host interactions mediated by miRNAs and their evolution in animals.
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影响因子：
64.8
作者：
Baek, Daehyun;Villen, Judit;Shin, Chanseok;Camargo, Fernando D.;Gygi, Steven P.;Bartel, David P.
通讯作者：
Bartel, David P.
影响因子：
10.5
作者：
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通讯作者：
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影响因子：
7.8
作者：
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通讯作者：
Slack, Frank J
影响因子：
4.1
作者：
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通讯作者：
Bonjardim, CA
影响因子：
56.9
作者：
Jopling, CL;Yi, MK;Sarnow, P
通讯作者：
Sarnow, P