Genome-Scale Assessment of Age-Related DNA Methylation Changes in Mouse Spermatozoa.
Genome-Scale Assessment of Age-Related DNA Methylation Changes in Mouse Spermatozoa.
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DOI：
10.1371/journal.pone.0167127
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Arima T
中科院分区：
文献类型：
--
作者：
Kobayashi N;Okae H;Hiura H;Chiba H;Shirakata Y;Hara K;Tanemura K;Arima T
DNA甲基化在精子的产生和功能中起着重要作用。最近的研究表明，精子中的DNA甲基化模式可以随着年龄的变化而变化，但对年龄相关的甲基化变化敏感的区域仍有待充分阐明。在这项研究中，我们进行了基因组规模的DNA甲基化谱从C57 BL/6 N小鼠获得的精子在8周（8周），18周（18周）和17个月（17个月）。18周和17个月样本之间的总体DNA甲基化模式没有实质性差异，除了17个月样本中长散布核元件的甲基化水平略有增加。我们发现，母亲甲基化印迹控制区（mICRs）和精子发生相关基因启动子在8周的样品中的甲基化水平比18周或17个月的样品高5-10%。单个序列读数的分析表明，这些区域在8 w精子的子集中完全甲基化（80-100%）。这些区域在出生后精原细胞的一个亚群中也被高度甲基化，这可能是8 w精子中DNA甲基化增加的来源。另一个可能的来源是体细胞的污染。尽管我们仔细纯化了精子，但很难完全排除体细胞污染的可能性。需要进一步的研究来澄清8周样本中DNA甲基化小幅增加的来源。总的来说，我们的研究结果表明，小鼠精子中的DNA甲基化模式在整个生殖生命中是相对稳定的。
DNA methylation plays important roles in the production and functioning of spermatozoa. Recent studies have suggested that DNA methylation patterns in spermatozoa can change with age, but the regions susceptible to age-related methylation changes remain to be fully elucidated. In this study, we conducted genome-scale DNA methylation profiling of spermatozoa obtained from C57BL/6N mice at 8 weeks (8w), 18 weeks (18w) and 17 months of age (17m). There was no substantial difference in the global DNA methylation patterns between 18w and 17m samples except for a slight increase of methylation levels in long interspersed nuclear elements in the 17m samples. We found that maternally methylated imprinting control regions (mICRs) and spermatogenesis-related gene promoters had 5–10% higher methylation levels in 8w samples than in 18w or 17m samples. Analysis of individual sequence reads suggested that these regions were fully methylated (80–100%) in a subset of 8w spermatozoa. These regions are also known to be highly methylated in a subset of postnatal spermatogonia, which might be the source of the increased DNA methylation in 8w spermatozoa. Another possible source was contamination by somatic cells. Although we carefully purified the spermatozoa, it was difficult to completely exclude the possibility of somatic cell contamination. Further studies are needed to clarify the source of the small increase in DNA methylation in the 8w samples. Overall, our findings suggest that DNA methylation patterns in mouse spermatozoa are relatively stable throughout reproductive life.
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DOI：
10.1098/rstb.2010.0026
发表时间：
2010-05-27
期刊：
Philosophical transactions of the Royal Society of London. Series B, Biological sciences
影响因子：
--
作者：
Phillips BT;Gassei K;Orwig KE
通讯作者：
Orwig KE
影响因子：
4.5
作者：
Kobayashi H;Sakurai T;Imai M;Takahashi N;Fukuda A;Yayoi O;Sato S;Nakabayashi K;Hata K;Sotomaru Y;Suzuki Y;Kono T
通讯作者：
Kono T
影响因子：
3.5
作者：
Kobayashi, Hisato;Sato, Akiko;Arima, Takahiro
通讯作者：
Arima, Takahiro
转录和烙印动力学在发展产后男性系干细胞中。
影响因子：
10.5
作者：
Hammoud SS;Low DH;Yi C;Lee CL;Oatley JM;Payne CJ;Carrell DT;Guccione E;Cairns BR
通讯作者：
Cairns BR
DOI：
10.1515/znc-1995-3-421
发表时间：
1995-03-01
期刊：
ZEITSCHRIFT FUR NATURFORSCHUNG C-A JOURNAL OF BIOSCIENCES
影响因子：
--
作者：
HACKERKLOM, UB
通讯作者：
HACKERKLOM, UB