Deep RNA sequencing reveals that microRNAs play a key role in lactation in rats.
Deep RNA sequencing reveals that microRNAs play a key role in lactation in rats.
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深度 RNA 测序揭示 microRNA 在大鼠哺乳中发挥关键作用。
DOI：
10.3945/jn.114.192575
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发表时间：
2014-08
影响因子：
4.2
通讯作者：
Chen, Hong
中科院分区：
文献类型：
--
作者：
Zhang, ChunLei;Zhao, YuLong;Wang, YanHong;Wu, Hui;Fang, XingTang;Chen, Hong
了解产妇生理学对哺乳困难的调节作用对母亲和婴儿都有好处。MicroRNAs（miRNAs）是一类非编码RNA，可能参与乳腺发育和功能的调控。在本研究中，使用深度RNA测序（RNA-seq）技术比较产后第1天和第7天（n = 1/d）大鼠（Rattus norvegicus）乳腺的2个合并RNA样本的miRNA和mRNA的表达谱，以鉴定可能控制泌乳限速步骤的关键miRNA及其靶基因。在产后第1天和第7天的大鼠乳腺样品中分别鉴定了总共395和400种已知的miRNA。与产后第1天相比，产后第7天有27个miRNAs表达差异。通过实时定量聚合酶链反应（qPCR）（n = 5）进一步分析哺乳期之间的表达差异。两个文库之间的rno-miR-30、rno-miR-1、rno-miR-145- 3 p、rno-miR-142、rno-miR-7a-5 p、rno-miR-3571、rno-miR-224- 5 p、rno-miR-362- 5 p、rno-miR-342 - 3 p、rno-miR-322 - 3 p、rno-miR-18 a-5 p和rno-miR-202- 5 p的ΔΔCt值在0.64至9.44之间变化;两个文库之间rno-miR-133、rno-miR-190 a-5 p、rno-miR-27 a-5 p、rno-miR-451- 5 p、rno-miR-3120、rno-miR-23 a-5 p、rno-miR-20 a-3 p、rno-miR-92 a-1- 5 p和rno-miR-134- 5 p的ΔΔCt值在-1.02至-4.37之间变化（P < 0.05）。分析了来自RNA-seq的表达mRNA基因与差异表达miRNA的推定靶基因（称为乳腺靶基因（MTG））的交叉。结果表明，2个基因集之间有1259个MTG重叠。通过实时定量PCR进一步证实了14个随机选择的MTG基因的表达（R（2）= 0.86，P < 0.01）。下调的MTG富含参与脂质生物合成的途径。该基因簇包括24个脂质代谢过程相关基因，这些基因是10个差异表达的miRNA的推定靶标。这些结果将有助于发现试图母乳喂养的妇女的哺乳性能差的生物学基础。
Understanding the regulatory contribution of maternal physiology to difficulties with lactation is beneficial to both mother and infant. MicroRNAs (miRNAs), a type of noncoding RNA, may be involved in the regulation of mammary gland development and function. In the present study, a deep RNA sequencing (RNA-seq) technique was used to compare the expression profile of miRNAs and mRNAs of 2 pooled RNA samples from day 1 and day 7 postpartum (n = 1/d) rat (Rattus norvegicus) mammary glands to identify key miRNAs and their target genes that may control the rate-limiting steps of lactation. A total of 395 and 400 known miRNAs were identified in days 1 and 7 postpartum rat mammary samples, respectively. Compared with day 1 postpartum, 27 miRNAs were differentially expressed at day 7 postpartum. The expression differences between lactation periods were further analyzed by real-time quantitative polymerase chain reaction (qPCR) (n = 5). The ΔΔCt values of rno-miR-30, rno-miR-1, rno-miR-145-3p, rno-miR-142, rno-miR-7a-5p, rno-miR-3571, rno-miR-224-5p, rno-miR-362-5p, rno-miR-342-3p, rno-miR-322-3p, rno-miR-18a-5p, and rno-miR-202-5p between the 2 libraries varied from 0.64 to 9.44; the ΔΔCt values of rno-miR-133, rno-miR-190a-5p, rno-miR-27a-5p, rno-miR-451-5p, rno-miR-3120, rno-miR-23a-5p, rno-miR-20a-3p, rno-miR-92a-1-5p, and rno-miR-134-5p between the 2 libraries varied from -1.02 to -4.37 (P < 0.05). The intersection of the expressed mRNA genes from RNA-seq and putative target genes of differentially expressed miRNAs, termed mammary gland target genes (MTGs), was analyzed. The results indicated that 1259 MTGs overlapped between the 2 gene sets. The expression of 14 randomly selected genes of the MTGs was further confirmed by real-time qPCR (R(2) = 0.86, P < 0.01). The downregulated MTGs were enriched for the pathways involved in lipid biosynthesis. This gene cluster included 24 lipid metabolic process-related genes, which were putative targets of 10 differentially expressed miRNAs. These results will be helpful in discovering the biologic underpinnings of poor lactation performance in women attempting to breastfeed.
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影响因子：
4.4
作者：
Li Z;Liu H;Jin X;Lo L;Liu J
通讯作者：
Liu J
影响因子：
9
作者：
通讯作者：
--
影响因子：
3.5
作者：
Can Yi;Wei Xie;Fu Li;Q. Lv;Jie He;Jiangbin Wu;D. Gu;Naihan Xu;Yaou Zhang
通讯作者：
Can Yi;Wei Xie;Fu Li;Q. Lv;Jie He;Jiangbin Wu;D. Gu;Naihan Xu;Yaou Zhang
DOI：
10.1073/pnas.1210057109
发表时间：
2012-10-16
影响因子：
11.1
作者：
Jabed, Anower;Wagner, Stefan;Laible, Goetz
通讯作者：
Laible, Goetz
影响因子：
11.2
作者：
Reddy SD;Ohshiro K;Rayala SK;Kumar R
通讯作者：
Kumar R