Effect of the herbal formulation Shen-Zhi-Ling on an APP/PS1 mouse model of Alzheimer's disease by modulating the biliverdin reductase/heme oxygenase 1 system.
Effect of the herbal formulation Shen-Zhi-Ling on an APP/PS1 mouse model of Alzheimer's disease by modulating the biliverdin reductase/heme oxygenase 1 system.
复制标题
中药参枝灵通过调节胆绿素还原酶/血红素加氧酶 1 系统对 APP/PS1 阿尔茨海默病小鼠模型的影响
DOI：
10.3892/etm.2017.4732
复制
发表时间：
2017-09
影响因子：
2.7
通讯作者：
Chi H
中科院分区：
文献类型：
--
作者：
Xing S;Shen D;Chen C;Wu B;Chi H
参脂灵（SZL）口服液是一种中药配方，主要用于临床治疗轻中度阿尔茨海默病（AD）。本研究的目的是探讨 SZL 治疗 AD 的效果和潜在机制。利用 APP/PS1 转基因小鼠评估 SZL 治疗（0.5 g/20 g/天）的效果。分别采用Morris水迷宫和硫磺素S染色分析评估认知障碍和β-淀粉样斑块，同时进行定量聚合酶链反应和蛋白质印迹分析来检测血红素加氧酶1（HO-1）和胆绿素还原酶（BVR）的mRNA和蛋白表达水平。此外，免疫荧光染色用于测量海马中的BVR和HO-1蛋白水平。目前的研究结果表明，SZL治疗能够改善记忆障碍并减少淀粉样斑块的积累，其改善作用可能归因于海马HO-1/BVR系统的调节。这些结果表明，SZL 可能是延缓 AD 发展的一种可能的补充和替代疗法。
Shen-Zhi-Ling (SZL) oral liquid is a traditional Chinese medicine formula that is mainly used for the clinical treatment of mild to moderate Alzheimer's disease (AD). The aim of the present study was to investigate the effects and underlying mechanisms of SZL treatment on AD. APP/PS1 transgenic mice were utilized to evaluate the effect of SZL treatment (0.5 g/20 g/day). Morris water maze and Thioflavin S staining analyses were used to evaluate the cognitive impairment and β-amyloid plaques, respectively, while quantitative polymerase chain reaction and western blot analysis were performed to examine the mRNA and protein expression levels of heme oxygenase 1 (HO-1) and biliverdin reductase (BVR). Furthermore, immunofluorescence staining was used to measure the BVR and HO-1 protein levels in the hippocampus. The findings of the current study demonstrated that SZL treatment was able to ameliorate the impairment of memory and reduce the accumulation of amyloid plaques, and its ameliorating effects may be attributed to the modulation of the HO-1/BVR system in the hippocampus. These results indicate that SZL may be a possible complementary and alternative therapy to delay the development of AD.
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影响因子：
3.7
作者：
Durairajan SS;Huang YY;Yuen PY;Chen LL;Kwok KY;Liu LF;Song JX;Han QB;Xue L;Chung SK;Huang JD;Baum L;Senapati S;Li M
通讯作者：
Li M
DOI：
10.1155/2014/913687
发表时间：
2014
期刊：
Evidence-based complementary and alternative medicine : eCAM
影响因子：
--
作者：
Pan W;Wang Q;Kwak S;Song Y;Qin B;Wang M;Yamamoto Y
通讯作者：
Yamamoto Y
影响因子：
2.5
作者：
Bi, Min;Tong, Suijun;Wang, Desheng
通讯作者：
Wang, Desheng
影响因子：
--
作者：
Perl, Daniel P.
通讯作者：
Perl, Daniel P.
DOI：
10.1073/pnas.0502173102
发表时间：
2005-05-17
影响因子：
11.1
作者：
Lerner-Marmarosh, N;Shen, J;Maines, MD
通讯作者：
Maines, MD