The relationship between the distribution of common carp and their environmental DNA in a small lake.
The relationship between the distribution of common carp and their environmental DNA in a small lake.
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DOI：
10.1371/journal.pone.0112611
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Sorensen PW
中科院分区：
文献类型：
--
作者：
Eichmiller JJ;Bajer PG;Sorensen PW
虽然环境DNA（eDNA）已被用于推断稀有水生物种的存在，但这项技术的许多方面仍然没有得到解决。特别是，eDNA和鱼类分布之间的关系尚不清楚。我们研究了鱼类的分布和它们的eDNA（检测率和浓度）在一个湖泊之间的关系。一个区域内的细胞色素B基因的普通鲤鱼（鲤鱼或“鲤鱼”），一种普遍存在的入侵鱼类，定量PCR（qPCR）测定，并用于测量eDNA在湖凝视（MN，美国），其中鲤鱼的密度和它们的分布已密切监测了几年。从湖中的22个地点采集了地表水、次地表水和沉积物样本，包括鲤鱼经常使用的区域。在水中，高鲤鱼使用的地区有较高的检测率和浓度的eDNA，但没有鱼的使用对沉积物eDNA的影响。eDNA在地表水和次地表水中的检出率和浓度差异均不显著（p≥0.5），表明eDNA在地表水中没有积累。检出率为高用水量>低用水量>沉积物。沉积物样品中的eDNA浓度高于检测限，按质量计比水高几个数量级，但检测限差导致检测率低。在我们研究的湖水中，eDNA的分布不均匀，这表明从水柱中去除eDNA的机制，如衰变和沉积，是迅速的。综上所述，这些结果表明，有效的eDNA采样方法应告知鱼类分布，eDNA浓度之间的差异显着小于100米的样品分开。
Although environmental DNA (eDNA) has been used to infer the presence of rare aquatic species, many facets of this technique remain unresolved. In particular, the relationship between eDNA and fish distribution is not known. We examined the relationship between the distribution of fish and their eDNA (detection rate and concentration) in a lake. A quantitative PCR (qPCR) assay for a region within the cytochrome b gene of the common carp (Cyprinus carpio or ‘carp’), an ubiquitous invasive fish, was developed and used to measure eDNA in Lake Staring (MN, USA), in which both the density of carp and their distribution have been closely monitored for several years. Surface water, sub-surface water, and sediment were sampled from 22 locations in the lake, including areas frequently used by carp. In water, areas of high carp use had a higher rate of detection and concentration of eDNA, but there was no effect of fish use on sediment eDNA. The detection rate and concentration of eDNA in surface and sub-surface water were not significantly different (p≥0.5), indicating that eDNA did not accumulate in surface water. The detection rate followed the trend: high-use water > low-use water > sediment. The concentration of eDNA in sediment samples that were above the limit of detection were several orders of magnitude greater than water on a per mass basis, but a poor limit of detection led to low detection rates. The patchy distribution of eDNA in the water of our study lake suggests that the mechanisms that remove eDNA from the water column, such as decay and sedimentation, are rapid. Taken together, these results indicate that effective eDNA sampling methods should be informed by fish distribution, as eDNA concentration was shown to vary dramatically between samples taken less than 100 m apart.
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影响因子：
4.4
作者：
Dell'Anno, A;Corinaldesi, C
通讯作者：
Corinaldesi, C
影响因子：
2
作者：
CROSS, DG;STOTT, B
通讯作者：
STOTT, B
影响因子：
1.9
作者：
Olson, Zachary H.;Briggler, Jeffrey T.;Williams, Rod N.
通讯作者：
Williams, Rod N.
在受控和现场实验中检测亚洲卡普的EDNA监视敏感性的验证。
影响因子：
3.7
作者：
Mahon AR;Jerde CL;Galaska M;Bergner JL;Chadderton WL;Lodge DM;Hunter ME;Nico LG
通讯作者：
Nico LG
影响因子：
2.2
作者：
Mumy, KL;Findlay, RH
通讯作者：
Findlay, RH