Validation of eDNA surveillance sensitivity for detection of Asian carps in controlled and field experiments.
Validation of eDNA surveillance sensitivity for detection of Asian carps in controlled and field experiments.
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在受控和现场实验中检测亚洲卡普的EDNA监视敏感性的验证。
DOI：
10.1371/journal.pone.0058316
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Nico LG
中科院分区：
文献类型：
--
作者：
Mahon AR;Jerde CL;Galaska M;Bergner JL;Chadderton WL;Lodge DM;Hunter ME;Nico LG
在北美的许多河流中，存在着多种非原生鲤科鱼类，包括青鱼（Mylpharyngodon piceus）、草鱼（Ctenopharyngodon idella）、鳙鱼（Hypophthalmichthys nobilis）、银鱼（Hypophthalmichthys molitrix）、鲤鱼（Cyprinus carpio）和金鱼（Carassius auratus）。所有这六个物种都发现在密西西比河流域和跟踪他们的入侵已被证明是困难的，特别是在丰度低。了解入侵前沿的位置对自然资源管理者很有价值，因为当人口较少时，可以最有效地防止未来的生态和经济损害。为了测试的准确性，环境DNA（eDNA）作为物种发生和相对丰度的早期指标，我们应用eDNA技术的6个非本地的鲤科鱼类物种pueridum目前在一个2.6河英里延伸的芝加哥（IL，美国）运河系统，随后用杀鱼剂处理。如中毒后回收的尸体数量所示，水样中检出阳性的比例随着六种物种的相对丰度而增加。报道了青鱼、草鱼和一种常见鲤鱼/金鱼的新标记，并提供了标记测试的细节以确保特异性。
In many North American rivers, populations of multiple species of non-native cyprinid fishes are present, including black carp (Mylpharyngodon piceus), grass carp (Ctenopharyngodon idella), bighead carp (Hypophthalmichthys nobilis), silver carp (Hypophthalmichthys molitrix), common carp (Cyprinus carpio), and goldfish (Carassius auratus). All six of these species are found in the Mississippi River basin and tracking their invasion has proven difficult, particularly where abundance is low. Knowledge of the location of the invasion front is valuable to natural resource managers because future ecological and economic damages can be most effectively prevented when populations are low. To test the accuracy of environmental DNA (eDNA) as an early indicator of species occurrence and relative abundance, we applied eDNA technology to the six non-native cyprinid species putatively present in a 2.6 river mile stretch of the Chicago (IL, USA) canal system that was subsequently treated with piscicide. The proportion of water samples yielding positive detections increased with relative abundance of the six species, as indicated by the number of carcasses recovered after poisoning. New markers for black carp, grass carp, and a common carp/goldfish are reported and details of the marker testing to ensure specificity are provided.
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影响因子：
2.2
作者：
Kocovsky, Patrick M.;Chapman, Duane C.;McKenna, James E.
通讯作者：
McKenna, James E.
影响因子：
8.3
作者：
Darling, John A.;Mahon, Andrew R.
通讯作者：
Mahon, Andrew R.
影响因子：
4.9
作者：
Lodge DM;Turner CR;Jerde CL;Barnes MA;Chadderton L;Egan SP;Feder JL;Mahon AR;Pfrender ME
通讯作者：
Pfrender ME
影响因子：
3.7
作者：
Takahara T;Minamoto T;Yamanaka H;Doi H;Kawabata Z
通讯作者：
Kawabata Z
影响因子：
3.7
作者：
Goldberg CS;Pilliod DS;Arkle RS;Waits LP
通讯作者：
Waits LP