Preparation of high-capacity strong cation exchange membrane for protein adsorption via surface-initiated atom transfer radical polymerization
Preparation of high-capacity strong cation exchange membrane for protein adsorption via surface-initiated atom transfer radical polymerization
复制标题
表面引发原子转移自由基聚合制备用于蛋白质吸附的高容量强阳离子交换膜
DOI：
10.1016/j.memsci.2012.09.053
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发表时间：
2013-01
影响因子：
9.5
通讯作者：
Wang, Chaozhan
中科院分区：
文献类型：
--
作者：
Wei, Yinmao;Ma, Jinju;Wang, Chaozhan
膜在生物分子的分离和纯化中具有越来越重要的作用。本工作采用表面引发原子转移自由基聚合法（SI-ATRP）制备了高通量强阳离子交换膜。首先将引发剂锚定到再生纤维素（RC）膜的表面，然后通过SI-ATRP将聚（4-苯乙烯磺酸钠）（聚（NASS））接枝到膜的表面上，得到具有磺酸盐作为交换基团的离子交换膜。通过改变ATRP反应时间来控制接枝率。衰减全反射傅里叶变换红外光谱（ATR-FTIR）和X射线光电子能谱（XPS）的结果表明，聚（NASS）成功地接枝到膜上。扫描电子显微镜（SEM）和原子力显微镜（AFM）表明，表面形貌表现出显着的变化，作为接枝反应的结果。水通量测试表明，表面改性在一定程度上减小了膜的孔径。以溶菌酶为模型蛋白，考察了改性膜在静态和动态条件下对溶菌酶的吸附性能。结果表明，随着ATRP反应时间的延长，蛋白质的吸附量增加，静态和动态吸附的最大吸附量分别达到138.3mg/mL和92.9mg/mL。
Membranes have an increasingly important niche in the isolation and purification of biomolecules. In this work, a strong cation exchange membrane with high capacity was prepared via surface-initiated atom transfer radical polymerization (SI-ATRP). An initiator was first anchored to the surface of a regenerated cellulose (RC) membrane, and then poly(sodium 4-styrenesulfonate) (poly(NASS)) was grafted onto the surface of the membrane via SI-ATRP, affording an ion exchange membrane with the sulfonate as the exchange group. The grafting degree was controlled by varying the ATRP reaction time. The results from attenuated total reflectance Fourier-transform infrared (ATR-FTIR) and X-ray photoelectron spectroscopy (XPS) indicated that poly(NASS) was successfully grafted onto the membrane. Scanning electron microscopy (SEM) and atomic force microscopy (AFM) demonstrated that the surface morphology exhibits a significant change as a result of the grafting reaction. The water flux measurement illustrated that the surface modification decreased the pore sizes of the membrane to some extent. Lysozyme was used as a model protein to evaluate the adsorption properties of the modified membranes under static and dynamic conditions. The results demonstrated that the protein adsorption capacities increased with increasing ATRP reaction time and could reach a maximum capacity of 138.3mg/mL and 92.9mg/mL for static and dynamic adsorption, respectively.
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DOI：
10.1016/j.memsci.2010.11.072
发表时间：
2011-03
期刊：
Fuel and Energy Abstracts
影响因子：
--
作者：
Ranran Feng;Chanchan Wang;Xiaochen Xu;Fenglin Yang;Guangjing Xu;T. Jiang
通讯作者：
Ranran Feng;Chanchan Wang;Xiaochen Xu;Fenglin Yang;Guangjing Xu;T. Jiang
影响因子：
9.9
作者：
Abu Seman, M. N.;Khayet, M.;Hilal, N.
通讯作者：
Hilal, N.
影响因子：
4.1
作者：
Staby, Arne;Jacobsen, Jan H.;Jensen, Inge Holm
通讯作者：
Jensen, Inge Holm
影响因子：
9.9
作者：
Chanchan Wang;Ranran Feng;Fenglin Yang
通讯作者：
Chanchan Wang;Ranran Feng;Fenglin Yang
影响因子：
9.5
作者：
A. H. M. Yusof;M. Ulbricht
通讯作者：
A. H. M. Yusof;M. Ulbricht