MicroInspector: a web tool for detection of miRNA binding sites in an RNA sequence.
MicroInspector: a web tool for detection of miRNA binding sites in an RNA sequence.
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DOI：
10.1093/nar/gki364
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发表时间：
2005-07-01
影响因子：
14.9
通讯作者：
Tabler, M
中科院分区：
文献类型：
--
作者：
Rusinov, V;Baev, V;Minkov, IN;Tabler, M
到目前为止，仅针对少数mRNA靶标，对MicroRNA（miRNA）的转录后基因表达（miRNA）调节。基于大量miRNA基因以及一个miRNA可能同时影响几个靶标的基因表达的可能性，预计核糖调节基因的数量将更高。在这里，我们描述了Web工具的微观检查员，该微检查将分析用户定义的RNA序列，该序列通常是mRNA或mRNA的一部分，用于出现已知和注册miRNA的结合位点。该程序允许温度变化，能量值的设置以及选择不同的miRNA数据库，以识别不同强度的miRNA结合位点。微观检查员可以在已知靶标mRNA中发现正确的miRNA交流位点。使用其他尚未描述这种相互作用的mRNA，我们经常发现具有相似质量的潜在miRNA结合位点，现在可以通过实验对其进行分析。 Microspordor程序易于使用，并且不需要特定的计算机技能。可以通过AT的Microspoctor Web服务器访问该服务。
Regulation of post-transcriptional gene expression by microRNAs (miRNA) has so far been validated for only a few mRNA targets. Based on the large number of miRNA genes and the possibility that one miRNA might influence gene expression of several targets simultaneously, the quantity of ribo-regulated genes is expected to be much higher. Here, we describe the web tool MicroInspector that will analyse a user-defined RNA sequence, which is typically an mRNA or a part of an mRNA, for the occurrence of binding sites for known and registered miRNAs. The program allows variation of temperature, the setting of energy values as well as the selection of different miRNA databases to identify miRNA-binding sites of different strength. MicroInspector could spot the correct sites for miRNA-interaction in known target mRNAs. Using other mRNAs, for which such an interaction has not yet been described, we discovered frequently potential miRNA binding sites of similar quality, which can now be analysed experimentally. The MicroInspector program is easy to use and does not require specific computer skills. The service can be accessed via the MicroInspector web server at .
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影响因子：
56.9
作者：
Lagos-Quintana, M;Rauhut, R;Tuschl, T
通讯作者：
Tuschl, T
影响因子：
1.8
作者：
HOFACKER, IL;FONTANA, W;SCHUSTER, P
通讯作者：
SCHUSTER, P
影响因子：
9.8
作者：
John B;Enright AJ;Aravin A;Tuschl T;Sander C;Marks DS
通讯作者：
Marks DS
影响因子：
14.9
作者：
Boutla, A;Delidakis, C;Tabler, M
通讯作者：
Tabler, M
影响因子：
7
作者：
Adai, A;Johnson, C;Sundaresan, V
通讯作者：
Sundaresan, V