Cytotoxic and pro-apoptotic effects of botanical drugs derived from the indigenous cultivated medicinal plant Paris polyphylla var. yunnanensis.
Cytotoxic and pro-apoptotic effects of botanical drugs derived from the indigenous cultivated medicinal plant Paris polyphylla var. yunnanensis.
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DOI：
10.3389/fphar.2023.1100825
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发表时间：
2023
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
背景：癌症是全球两大主要死亡原因之一。民族植物学研究是以原住民的历史经验和实践为“原型”，发现有效抗癌药物的方法之一。巴黎Polyphylla var. yunnanensis（Franch.）手。马兹已经被中国云南的彝族，白族，傣族和纳西族用作治疗癌症的植物药，在那里本种在云南大规模地被广泛栽培。 材料与方法：为从民间医药知识中鉴定具有抗癌活性的物质，采用色谱法从多叶连翘根茎中分离皂苷类成分。对分离得到的6个皂苷类化合物进行了结构测定。采用MTS法对5种提取物和6种纯化合物进行细胞毒性评价。何首乌总皂苷的含量测定。采用高效液相色谱法对云南茶进行了分析。采用细胞周期分析、细胞凋亡分析、线粒体膜电位测定等方法评价其促凋亡活性。 结果如下：结果表明，5种提取物和6种纯皂苷对3种人肝癌细胞株（SMMC-7721、HepG 2和SK-HEP-1）和1种非小细胞肺癌细胞株（A549）具有明显的细胞毒活性。重楼根茎中巴黎皂苷I、II和VII的含量为6.96%。结果表明，云南甘草中甘草酸的含量高于中国药典标准（0.6%）。六种皂苷诱导三种人癌细胞系（A549、SMMC-7721和HepG 2）显著的凋亡和细胞周期阻滞，这与线粒体膜电位的丧失有关。 结论：本研究结果支持栽培多叶连翘的可行性。根据云南民族药的知识，云南白药有可能成为一种替代野生资源的抗癌药物。
Background: Cancer is one of the top two leading causes of death worldwide. Ethnobotanical research, it is one of methods, which is able to discover effective anticancer drugs based on “prototype” of indigenous people’s historical experiences and practices. The rhizomes of Paris polyphylla var. yunnanensis (Franch.) Hand.-Mazz. have been used as botanical drugs to treat cancer by Yi, Bai, Dai, and Naxi ethnic groups in Yunnan, China, where this species is widely cultivated in a large scale in Yunnan. Materials and methods: To identify the substances of anticancer activities based on indigenous medicine knowledge, chromatography was performed to separate saponins from the rhizomes of P. polyphylla var. yunnanensis, followed by spectroscopy to determine the structure of six isolated saponins. The cytotoxicity of five extracts and six pure compounds were evaluated by MTS method. Quantitative determination of total saponins of P. polyphylla var. yunnanensis was analyzed by HPLC. Cell cycle assay, apoptosis assay, and mitochondrial membrane potential were used to evaluate the pro-apoptotic activity in vitro. Results: Five extracts and six pure saponins showed significant inhibitory cytotoxic activities of three human liver cancer cell lines (SMMC-7721, HepG2, and SK-HEP-1) and one non-small-cell lung cancer cell line (A549). The contents of Paris saponins I, II, and VII were 6.96% in the rhizomes of P. polyphylla var. yunnanensis, much higher than Chinese Pharmacopoeia standards (0.6%). Six saponins induced significant apoptosis and cell cycle arrest in three human cancer cell lines (A549, SMMC-7721, and HepG2), which was associated with the loss of mitochondrial membrane potential. Conclusion: The result of this study support that cultivated P. polyphylla var. yunnanensis could be a substitute for wild resource as an anticancer medicine based on indigenous medicine knowledge.
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影响因子：
1.2
作者：
Chaudhari, Atul A.;Seol, Jae-Won;Park, Sang-Youel
通讯作者：
Park, Sang-Youel
影响因子：
16.2
作者：
CORY, AH;OWEN, TC;CORY, JG
通讯作者：
CORY, JG
影响因子：
5.1
作者：
Kang, Li-Ping;Liu, Yi-Xun;Ma, Bai-Ping
通讯作者：
Ma, Bai-Ping
影响因子：
2.2
作者：
Castedo, M;Ferri, K;Kroemer, G
通讯作者：
Kroemer, G
影响因子：
5.4
作者：
Foo, Jhi Biau;Yazan, Latifah Saiful;Abdullah, Rasedee
通讯作者：
Abdullah, Rasedee