A widespread bacterial type VI secretion effector superfamily identified using a heuristic approach.
A widespread bacterial type VI secretion effector superfamily identified using a heuristic approach.
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DOI：
10.1016/j.chom.2012.04.007
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发表时间：
2012-05-17
影响因子：
30.3
通讯作者：
Mougous JD
中科院分区：
文献类型：
--
作者：
Russell AB;Singh P;Brittnacher M;Bui NK;Hood RD;Carl MA;Agnello DM;Schwarz S;Goodlett DR;Vollmer W;Mougous JD
采用复杂的机制来促进界面细菌之间的信息交换。铜绿假单胞菌的VI型分泌系统（T6SS）显示出将细胞壁靶向效应物递送至邻近细胞。然而，溶菌效应的一般性，而且，抗菌T6S，仍然未知。使用来自实验验证的细菌T6SS效应子和信息学的参数，我们确定了T6SS相关肽聚糖降解效应子的遗传分散超家族。效应子分为四个家族，由不同特异性的肽聚糖酰胺酶组成。效应子严格地与同源免疫蛋白共同出现，表明自身中毒是抗菌T6SS的一般性质，并且通过系统的效应子递送在自然界中施加了强选择性压力。抗菌效应物存在于过多的生物体中，包括许多栖息或感染人体内多微生物小生境的生物体，表明该系统可以介导环境和临床意义的细菌间相互作用。
Sophisticated mechanisms are employed to facilitate information exchange between interfacing bacteria. A type VI secretion system (T6SS) of Pseudomonas aeruginosa was shown to deliver cell wall-targeting effectors to neighboring cells. However, the generality of bacteriolytic effectors, and moreover, of antibacterial T6S, remained unknown. Using parameters derived from experimentally validated bacterial T6SS effectors and informatics, we identified a phylogenetically disperse superfamily of T6SS-associated peptidoglycan-degrading effectors. The effectors separate into four families composed of peptidoglycan amidase enzymes of differing specificities. Effectors strictly co-occur with cognate immunity proteins, indicating that self-intoxication is a general property of antibacterial T6SSs and effector delivery by the system exerts a strong selective pressure in nature. The presence of antibacterial effectors in a plethora of organisms, including many that inhabit or infect polymicrobial niches in the human body, suggests that the system could mediate interbacterial interactions of both environmental and clinical significance.
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影响因子：
1.5
作者：
Cohan, FM
通讯作者：
Cohan, FM
影响因子：
30.3
作者：
Jani AJ;Cotter PA
通讯作者：
Cotter PA
影响因子：
64.8
作者：
通讯作者：
--
影响因子：
6.7
作者：
Li M;Le Trong I;Carl MA;Larson ET;Chou S;De Leon JA;Dove SL;Stenkamp RE;Mougous JD
通讯作者：
Mougous JD
影响因子：
11.4
作者：
Cava, Felipe;de Pedro, Miguel A.;Waldor, Matthew K.
通讯作者：
Waldor, Matthew K.