Binding of nitrate to renal brush border membranes studied with 14N nuclear magnetic resonance (NMR).
Binding of nitrate to renal brush border membranes studied with 14N nuclear magnetic resonance (NMR).
复制标题
使用 14N 核磁共振 (NMR) 研究硝酸盐与肾刷状缘膜的结合。
DOI：
10.1016/0005-2736(95)00070-j
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Arruda,JA
中科院分区：
文献类型：
--
作者：
Galanter,WL;Ruiz,OS;Labotka,RJ;Arruda,JA
肾刷状缘膜含有多种阴离子交换剂，可能在电解质运输和 pH 调节中发挥作用。为了帮助表征存在的交换剂类型和这些膜的结合特性，研究了硝酸盐 (NO3−) 与高度纯化的兔肾刷状缘膜囊泡的结合。该方法基于硝酸盐 14 N-NMR 信号的结合诱导四极弛豫 [1,2]。刷状缘膜囊泡引起14 N-NMR硝酸盐信号的弛豫，其特征在于相对高的亲和力位点，KD=6.7±1.5mM，以及与膜的非特异性相互作用，KD>150mM。由于高亲和力结合位点，阴离子转运抑制剂 4,4'-二硝基芪-2,2'-二磺酸盐 (DNDS) 可抑制 51 ± 6% (n = 4) 的弛豫。 DNDS 抑制可通过 Kiof 10-80 μM 来表征。发现碳酸氢盐和甲酸盐 (HCO2−) 都部分抑制高亲和力诱导的松弛，最大抑制分别为 37 ± 8% (n = 3) 和 30 ± 2% (n = 3)。饱和浓度的碳酸氢盐和甲酸盐的抑制作用是非相加的，这表明存在可以结合硝酸盐以及碳酸氢盐和甲酸盐的二苯乙烯敏感交换剂。这项研究表明了这种新方法对于进一步研究这些膜和其他膜上的阴离子交换剂的有用性。
Renal brush border membranes contain several anion exchangers that may play a role in electrolyte transport and pH regulation. To help characterize the types of exchangers present and the binding properties of these membranes, the binding of nitrate (NO3−) to highly purified rabbit kidney brush border membrane vesicles was studied. The method is based on the binding induced quadrupole relaxation of the14N-NMR signal of nitrate [1,2]. Brush border membrane vesicles caused a relaxation of the14N-NMR nitrate signal which could be characterized by relatively high affinity sites, KD= 6.7 ± 1.5 mM, as well as nonspecific interactions with the membranes, KD> 150 mM. The anion transport inhibitor 4,4′-dinitrostilbene-2,2′-disulfonate (DNDS) inhibited 51 ± 6% (n = 4) of the relaxation due to the high affinity binding sites. The DNDS inhibition could be characterized by a Kiof 10–80 μM. Both bicarbonate and formate (HCO2−) were found to partially inhibit the high affinity induced relaxation, with maximal inhibition of 37 ± 8% (n = 3) and 30 ± 2% (n = 3), respectively. The inhibitory effects of saturating concentrations of bicarbonate and formate were non-additive, suggesting the existence of a stilbene sensitive exchanger that can bind nitrate, as well as both bicarbonate and formate. This study indicates the usefulness of this new method for further investigation of anion exchangers on these and other membranes.
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抑制剂对兔肾刷状缘膜囊泡阴离子交换剂的影响。
DOI：
--
发表时间：
1994
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
McConnell,KR;Aronson,PS
通讯作者：
Aronson,PS
氯化物与人氧合血红蛋白和脱氧血红蛋白结合的核磁共振四极弛豫研究。
影响因子：
5.6
作者：
E. Chiancone;J. Norne;S. Forsén;E. Antonini;J. Wyman
通讯作者：
J. Wyman
影响因子：
19.6
作者：
P. Wistrand;K. Knuuttila
通讯作者：
K. Knuuttila
碳酸酐酶的温度和竞争性阴离子结合研究。
影响因子：
3.9
作者：
R. Ward;M. Cull
通讯作者：
M. Cull
核磁共振波谱学的多核方法
DOI：
--
发表时间：
1983
期刊：
影响因子：
--
作者：
J. B. Lambert;F. Riddell
通讯作者：
F. Riddell