The RNA Chaperone Protein Hfq Regulates the Characteristic Sporulation and Insecticidal Activity of Bacillus thuringiensis.
The RNA Chaperone Protein Hfq Regulates the Characteristic Sporulation and Insecticidal Activity of Bacillus thuringiensis.
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DOI：
10.3389/fmicb.2022.884528
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发表时间：
2022
影响因子：
5.2
通讯作者：
中科院分区：
文献类型：
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作者：
苏云金芽孢杆菌（Bt）是目前应用最广泛的生物杀虫剂之一。在生长和产孢过程中能产生多种毒力因子和杀虫晶体蛋白。另一方面，Hfq是一种细菌RNA分子伴侣，可以调节不同种类RNA的功能，从而影响各种细菌表型。为进一步研究Bt中Hfq基因的生理功能，以BMB171为出发菌株，采用不同组合敲除其基因组中的1个、2个和3个hfq基因，比较敲除突变株与出发菌株的表型差异。我们确实观察到了几种表型的显著变化，包括运动性、生物膜形成、孢子形成和对棉铃虫的杀虫活性等。随后，我们通过转录组研究发现，当所有hfq基因被删除时，Bt中32.5%的基因发生了差异转录，尤其是与孢子形成相关和毒力相关的基因发生了变化。以上结果表明，Hfq在Bt中起着关键作用，可以调节Bt的多种生理功能。研究Bt中Hfq的调控机制，特别是挖掘其产孢和杀虫活性的调控网络，为更好地利用Bt作为高效杀虫剂奠定理论基础。
Bacillus thuringiensis (Bt) is one of the most widely used bio-insecticides at present. It can produce many virulence factors and insecticidal crystal proteins during growth and sporulation. Hfq, on the other hand, is a bacterial RNA chaperone that can regulate the function of different kinds of RNAs, thereby affecting various bacterial phenotypes. To further explore the physiological functions of Hfq in Bt, we took BMB171 as the starting strain, knocked out one, two, or three hfq genes in its genome in different combinations, and compared the phenotypic differences between the deletion mutant strains and the starting strain. We did observe significant changes in several phenotypes, including motility, biofilm formation, sporulation, and insecticidal activity against cotton bollworm, among others. Afterward, we found through transcriptome studies that when all hfq genes were deleted, 32.5% of the genes in Bt were differentially transcribed, with particular changes in the sporulation-related and virulence-related genes. The above data demonstrated that Hfq plays a pivotal role in Bt and can regulate its various physiological functions. Our study on the regulatory mechanism of Hfq in Bt, especially the mining of the regulatory network of its sporulation and insecticidal activity, could lay a theoretical foundation for the better utilization of Bt as an effective insecticide.
影响因子：
3.7
作者：
Hämmerle H;Beich-Frandsen M;Večerek B;Rajkowitsch L;Carugo O;Djinović-Carugo K;Bläsi U
通讯作者：
Bläsi U