Immunomodulatory differences between mesenchymal stem cells from different oral tissues.
Immunomodulatory differences between mesenchymal stem cells from different oral tissues.
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DOI：
10.1016/j.heliyon.2023.e23317
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发表时间：
2024-01-15
期刊：
影响因子：
4
通讯作者：
Kaigler, Darnell
中科院分区：
文献类型：
--
作者：
Cao, Chen;Maska, Bartosz;Malik, Malika A.;Tagett, Rebecca;Kaigler, Darnell
关键词：
间充质干细胞(MSCs)最近被发现具有潜在的治疗免疫调节特性。从不同口腔组织中分离的MSCs具有相似的形态和免疫表型，但尚未对其基因表达和免疫调节特性进行直接比较。我们从同一患者中分离出牙槽骨源性MSCs(ABMSCs)、牙髓干细胞(DPSCs)和牙周源性MSCs(GMSCs)，并比较它们的免疫表型和转录本。此外，我们比较了它们与THP-1人单核细胞共培养时产生的可溶性免疫调节细胞因子以及它们的免疫调节特性。RNA测序显示DPSCs基因表达差异显著，而aBMSCs和GMSCs差异表达基因较少。与aBMSCs和GMSCs相比，DPSCs的骨桥蛋白分泌也明显减少。最后，DPSCS对THP-1细胞的免疫抑制作用没有达到aBMSCs和GMSCs的程度。这些发现表明，来自不同口腔组织的MSCs具有不同的转录和免疫调节特性。
Mesenchymal stem cells (MSCs) have recently been identified as having potentially therapeutic immunomodulatory properties. MSCs isolated from different oral tissues have similar morphology and immunophenotypes, however, direct comparisons of their gene expression and immunomodulatory properties have not been conducted. We isolated alveolar bone-derived MSCs (aBMSCs), dental pulp stem cells (DPSCs) and gingiva-derived MSCs (GMSCs) from the same patients and compared their immunophenotypes and transcriptomes. Additionally, we compared their production of soluble immunomodulatory cytokines as well as their immunoregulatory properties in coculture with THP-1 human monocytic cells. RNA sequencing revealed distinct gene expression in DPSCs while aBMSCs and GMSCs had less differentially expressed genes. DPSCs also had significantly less secretion of osteopontin compared to aBMSCs and GMSCs. Finally, DPSCs did not exhibit an immunosuppresive effect on THP-1 cells to the same degree as aBMSCs and GMSCs. These findings demonstrate that MSCs from different oral tissues have distinct transcriptomes and immunoregulatory properties.
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影响因子：
2.6
作者：
Kim, Jaehyup;Hematti, Peiman
通讯作者：
Hematti, Peiman
影响因子：
32.4
作者：
Ashcroft, AJ;Cruickshank, SM;Carding, SR
通讯作者：
Carding, SR
影响因子：
4.4
作者：
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通讯作者：
Navarrete, Cristina V.
影响因子：
5.2
作者：
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通讯作者：
Kanhai, HHH
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y