The Exon Junction Complex Controls the Efficient and Faithful Splicing of a Subset of Transcripts Involved in Mitotic Cell-Cycle Progression.
The Exon Junction Complex Controls the Efficient and Faithful Splicing of a Subset of Transcripts Involved in Mitotic Cell-Cycle Progression.
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DOI：
10.3390/ijms17081153
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发表时间：
2016-08-02
影响因子：
5.6
通讯作者：
Inoue K
中科院分区：
文献类型：
--
作者：
Fukumura K;Wakabayashi S;Kataoka N;Sakamoto H;Suzuki Y;Nakai K;Mayeda A;Inoue K
外显子连接复合物（exon junction complex，EJC）沉积在外显子-外显子连接上游的剪接mRNA上，在多种剪接后基因表达事件中发挥重要作用，如mRNA输出、监视、定位和翻译。然而，人EJC在前mRNA剪接中的直接作用尚未完全了解。使用HeLa细胞，我们耗尽了EJC核心组分之一Y14，并通过深度测序（RNA-Seq）分析了所得的转录组，并通过RT-PCR进行了确认。我们发现，Y14是一组转录本的有效和忠实剪接所必需的，该组转录本富含参与有丝分裂细胞周期进程的含短内含子的基因。EJC核心组分（Y14、eIF 4AIII或MAGOH）与携带短内含子的模型报告子前mRNA的拴系表明，这些核心组分是剪接激活的先决条件。综上所述，我们得出结论，EJC核心组装前mRNA的有丝分裂细胞周期相关基因的短内含子的特定子集的有效和忠实的剪接是至关重要的。
The exon junction complex (EJC) that is deposited onto spliced mRNAs upstream of exon–exon junctions plays important roles in multiple post-splicing gene expression events, such as mRNA export, surveillance, localization, and translation. However, a direct role for the human EJC in pre-mRNA splicing has not been fully understood. Using HeLa cells, we depleted one of the EJC core components, Y14, and the resulting transcriptome was analyzed by deep sequencing (RNA-Seq) and confirmed by RT–PCR. We found that Y14 is required for efficient and faithful splicing of a group of transcripts that is enriched in short intron-containing genes involved in mitotic cell-cycle progression. Tethering of EJC core components (Y14, eIF4AIII or MAGOH) to a model reporter pre-mRNA harboring a short intron showed that these core components are prerequisites for the splicing activation. Taken together, we conclude that the EJC core assembled on pre-mRNA is critical for efficient and faithful splicing of a specific subset of short introns in mitotic cell cycle-related genes.
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影响因子：
10.5
作者：
Ideue, Takashi;Sasaki, Yasnory T. F.;Hirose, Tetsuro
通讯作者：
Hirose, Tetsuro
影响因子：
14.9
作者：
Fukumura, Kazuhiro;Kato, Ayako;Inoue, Kunio
通讯作者：
Inoue, Kunio
影响因子：
4.8
作者：
McCracken, S;Longman, D;Blencowe, BJ
通讯作者：
Blencowe, BJ
DOI：
10.1016/0167-4781(94)90060-4
发表时间：
1994-10-18
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION
影响因子：
--
作者：
NOTHWANG, HG;TAMURA, T;ICHIHARA, A
通讯作者：
ICHIHARA, A
影响因子：
16.8
作者：
Murachelli, Andrea Giovanni;Ebert, Judith;Conti, Elena
通讯作者：
Conti, Elena