Enhanced matrix production by cocultivated human stem cells and chondrocytes under concurrent mechanical strain.
Enhanced matrix production by cocultivated human stem cells and chondrocytes under concurrent mechanical strain.
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在同时机械应变下通过共培养的人干细胞和软骨细胞增强基质产生。
DOI：
10.1007/s11626-021-00592-4
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发表时间：
2021-06
期刊：
影响因子：
--
通讯作者：
Van Wie BJ
中科院分区：
文献类型：
--
作者：
Abusharkh HA;Mallah AH;Amr MM;Mendenhall J;Gozen BA;Tingstad EM;Abu-Lail NI;Van Wie BJ
骨关节炎的常规治疗未能重建功能性关节软骨。骨关节炎的组织工程临床治疗，包括自体软骨细胞植入，通过注射细胞悬液填充骨关节炎膝关节软骨内的病变，提供了一种替代方法。软骨细胞植入的成功依赖于软骨细胞系的可用性，以及它们对高机械负荷的弹性。我们假设，我们可以通过补充培养人脂肪源性干细胞来减少治疗所需的人关节软骨细胞的数量，当暴露于高机械负荷时，干细胞将对混合培养物具有保护和刺激作用，并且共培养将增强所需细胞外基质蛋白的产生，而不是单独拉伸软骨细胞产生的蛋白。在这项工作中，脂肪来源的干细胞和关节软骨细胞分别培养或共培养的比例为3：1，1：1，和1：3在静态板或过度循环拉伸应变10%和结果进行了比较，培养的两种细胞类型单独与和没有循环应变。结果表明，工程化关节软骨中75%的软骨细胞可以被干细胞替代，在所有培养条件下胶原蛋白和糖胺聚糖含量都高于拉伸的软骨细胞培养物。这可以在没有观察到对细胞活力的不利影响的情况下进行。胶原蛋白和糖胺聚糖的分泌，当与单独的软骨细胞相比，在10%的应变，分别增强6.1和2倍，软骨细胞与干细胞以1：3的比例共培养。
Conventional treatments of osteoarthritis have failed to rebuild functional articular cartilage. Tissue engineering clinical treatments for osteoarthritis, including autologous chondrocyte implantation, provides an alternative approach by injecting a cell suspension to fill lesions within the cartilage in osteoarthritic knees. The success of chondrocyte implantation relies on the availability of chondrogenic cell lines, and their resilience to high mechanical loading. We hypothesize we can reduce the numbers of human articular chondrocytes necessary for a treatment by supplementing cultures with human adipose-derived stem cells, that stem cells will have protective and stimulatory effects on mixed cultures when exposed to high mechanical loads, and that coculture will enhance production of requisite extracellular matrix proteins over those produced by stretched chondrocytes alone. In this work, adipose-derived stem cells and articular chondrocytes were cultured separately or cocultivated at ratios of 3:1, 1:1, and 1:3 in static plates or under excessive cyclic tensile strain of 10% and results were compared to culturing of both cell types alone with and without cyclic strain. Results indicate 75% of chondrocytes in engineered articular cartilage can be replaced with stem cells with enhanced collagen over all culture conditions and glycosaminoglycan content over stretched cultures of chondrocytes. This can be done without observing adverse effects on cell viability. Collagen and glycosaminoglycan secretion, when compared to chondrocyte alone under 10% strain, were enhanced 6.1- and 2-fold, respectively, by chondrocytes cocultivated with stem cells at a ratio of 1:3.
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DOI：
10.1097/01.mco.0000068964.34812.2b
发表时间：
2003-05-01
影响因子：
3.1
作者：
Deschner, J;Hofman, CR;Agarwal, S
通讯作者：
Agarwal, S
影响因子：
--
作者：
Lawrence, Reva C.;Felson, David T.;Wolfe, Frederick
通讯作者：
Wolfe, Frederick
机械应力对软骨细胞表型和软骨细胞细胞外基质表达的影响
影响因子：
4.6
作者：
Liu Q;Hu X;Zhang X;Duan X;Yang P;Zhao F;Ao Y
通讯作者：
Ao Y
影响因子：
3.3
作者：
de Windt, Tommy S.;Sorel, Juliette C.;Saris, Daniel B. F.
通讯作者：
Saris, Daniel B. F.
影响因子：
4.3
作者：
Liu, Yubao;Lin, Lupan;Lin, Fuqing
通讯作者：
Lin, Fuqing