Effects of mechanical stress on chondrocyte phenotype and chondrocyte extracellular matrix expression.
Effects of mechanical stress on chondrocyte phenotype and chondrocyte extracellular matrix expression.
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机械应力对软骨细胞表型和软骨细胞细胞外基质表达的影响
DOI：
10.1038/srep37268
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发表时间：
2016-11-17
影响因子：
4.6
通讯作者：
Ao Y
中科院分区：
文献类型：
--
作者：
Liu Q;Hu X;Zhang X;Duan X;Yang P;Zhao F;Ao Y
机械因素在调节骨关节炎软骨退化的发展中起着关键作用。本研究旨在确定机械应力对软骨和软骨细胞的影响。为了探讨机械应力对软骨形态的影响，我们通过组织学和显微镜检查观察了不同区域的软骨。进行纳米压痕以评估软骨生物力学。为了研究机械应力对软骨细胞的影响，使用 Flexcell-5000 对人关节软骨细胞的单层培养物施加循环拉伸应变（CTS，0.5Hz，10%）。我们通过原子力显微镜量化了软骨细胞的机械特性。暴露于 CTS 后，软骨细胞用甲苯胺蓝和阿新蓝染色。 CTS 后通过 qPCR 和免疫荧光分析检测软骨细胞中细胞外基质 (ECM) 分子的表达。我们的结果表明不同软骨区域具有不同的形态和机械特性。总之，机械应力可以影响软骨细胞表型，从而改变软骨细胞ECM的表达。
Mechanical factors play a key role in regulating the development of cartilage degradation in osteoarthritis. This study aimed to identify the influence of mechanical stress in cartilage and chondrocytes. To explore the effects of mechanical stress on cartilage morphology, we observed cartilages in different regions by histological and microscopic examination. Nanoindentation was performed to assess cartilage biomechanics. To investigate the effects of mechanical stress on chondrocytes, cyclic tensile strain (CTS, 0.5 Hz, 10%) was applied to monolayer cultures of human articular chondrocytes by using Flexcell-5000. We quantified the mechanical properties of chondrocytes by atomic force microscopy. Chondrocytes were stained with Toluidine blue and Alcian blue after exposure to CTS. The expression of extracellular matrix (ECM) molecules was detected by qPCR and immunofluorescence analyses in chondrocytes after CTS. Our results demonstrated distinct morphologies and mechanical properties in different cartilage regions. In conclusion, mechanical stress can affect the chondrocyte phenotype, thereby altering the expression of chondrocyte ECM.
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影响因子：
5.6
作者：
Pascarelli NA;Collodel G;Moretti E;Cheleschi S;Fioravanti A
通讯作者：
Fioravanti A
影响因子：
7
作者：
Leipzig, N. D.;Eleswarapu, S. V.;Athanasiou, K. A.
通讯作者：
Athanasiou, K. A.
影响因子：
7
作者：
Kawakita, K.;Nishiyama, T.;Kurosaka, M.
通讯作者：
Kurosaka, M.
影响因子：
3.7
作者：
Chahine NO;Blanchette C;Thomas CB;Lu J;Haudenschild D;Loots GG
通讯作者：
Loots GG
影响因子：
--
作者：
Agarwal, S;Deschner, J;Piesc, N
通讯作者：
Piesc, N