Strain, Soil-Type, Irrigation Regimen, and Poultry Litter Influence Salmonella Survival and Die-off in Agricultural Soils.
Strain, Soil-Type, Irrigation Regimen, and Poultry Litter Influence Salmonella Survival and Die-off in Agricultural Soils.
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DOI：
10.3389/fmicb.2021.590303
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发表时间：
2021
影响因子：
5.2
通讯作者：
Strawn LK
中科院分区：
文献类型：
--
作者：
Bardsley CA;Weller DL;Ingram DT;Chen Y;Oryang D;Rideout SL;Strawn LK
使用未经处理的动物源性生物土壤改良剂（BSAAO）已被确定为沙门氏菌在农产品生长环境中传播和持续存在的一种潜在机制。因此，需要有关影响土壤中沙门氏菌浓度的因素的数据。这里的目的是（i）比较死亡12沙门氏菌菌株接种后，在修改的土壤和（ii）表征任何显着的影响与土壤类型，灌溉方案，并修改沙门氏菌的生存和死亡。使用随机完全区组设计进行三个温室试验。将每种菌株（~4 log CFU/g）与改良或未改良的砂壤土或粘壤土均质化。在接种后0、0.167（4 h）、1、2、4、7、10、14、21、28、56、84、112、168、210、252和336天（dpi）或直至两个连续样品为富集阴性，对25 g样品中的沙门氏菌水平进行计数。在菌株、土壤类型、灌溉和（i）最后检测时间（存活率）和（ii）每个时间点的浓度（死亡率）之间进行回归分析。应变，灌溉，土壤类型，和修正案的类似影响，确定了生存和死亡的模型。菌株解释了高达18%的生存方差，高达19%的死亡率的方差。沙门氏菌平均存活129天，在改良土壤中，然而，沙门氏菌存活，平均30天以上的粘土壤土比砂壤土[95%置信区间（CI）= 45，15]，与存活时间范围从84到210天的个别菌株在日常灌溉。当使用回归树研究菌株特异性关联时，S。Javiana和S. Saintpaul在砂壤土中存活时间较长，而大多数其他菌株在粘壤土中存活时间较长。沙门氏菌也存活，平均128天以上，每周灌溉时，每天（CI = 101，154），和89天以上的修正土壤，比非修正土壤（CI = 61，116）。总体而言，这项研究提供了深入了解沙门氏菌的生存后，污染的领域土壤BSAAO。具体而言，沙门氏菌的存活可能是菌株特异性的，受到土壤特性和管理实践的影响。这些数据有助于风险评估和菌株选择，以用于挑战和验证研究。
The use of untreated biological soil amendments of animal origin (BSAAO) have been identified as one potential mechanism for the dissemination and persistence of Salmonella in the produce growing environment. Data on factors influencing Salmonella concentration in amended soils are therefore needed. The objectives here were to (i) compare die-off between 12 Salmonella strains following inoculation in amended soil and (ii) characterize any significant effects associated with soil-type, irrigation regimen, and amendment on Salmonella survival and die-off. Three greenhouse trials were performed using a randomized complete block design. Each strain (~4 log CFU/g) was homogenized with amended or non-amended sandy-loam or clay-loam soil. Salmonella levels were enumerated in 25 g samples 0, 0.167 (4 h), 1, 2, 4, 7, 10, 14, 21, 28, 56, 84, 112, 168, 210, 252, and 336 days post-inoculation (dpi), or until two consecutive samples were enrichment negative. Regression analysis was performed between strain, soil-type, irrigation, and (i) time to last detect (survival) and (ii) concentration at each time-point (die-off rate). Similar effects of strain, irrigation, soil-type, and amendment were identified using the survival and die-off models. Strain explained up to 18% of the variance in survival, and up to 19% of variance in die-off rate. On average Salmonella survived for 129 days in amended soils, however, Salmonella survived, on average, 30 days longer in clay-loam soils than sandy-loam soils [95% Confidence interval (CI) = 45, 15], with survival time ranging from 84 to 210 days for the individual strains during daily irrigation. When strain-specific associations were investigated using regression trees, S. Javiana and S. Saintpaul were found to survive longer in sandy-loam soil, whereas most of the other strains survived longer in clay-loam soil. Salmonella also survived, on average, 128 days longer when irrigated weekly, compared to daily (CI = 101, 154), and 89 days longer in amended soils, than non-amended soils (CI = 61, 116). Overall, this study provides insight into Salmonella survival following contamination of field soils by BSAAO. Specifically, Salmonella survival may be strain-specific as affected by both soil characteristics and management practices. These data can assist in risk assessment and strain selection for use in challenge and validation studies.
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影响因子：
2.8
作者：
Alali, Walid Q.;Thakur, Siddhartha;Gebreyes, Wondwossen A.
通讯作者：
Gebreyes, Wondwossen A.
影响因子：
2.4
作者：
Holley, Richard A.;Arrus, Katia M.;Blank, Gregory
通讯作者：
Blank, Gregory
肠道沙门氏菌：血清射手之间的生存，定殖和毒力差异。
影响因子：
--
作者：
Andino A;Hanning I
通讯作者：
Hanning I
影响因子：
2
作者：
Chinivasagam, H. N.;Redding, M.;Blackall, P. J.
通讯作者：
Blackall, P. J.
影响因子：
5.2
作者：
Bell, Rebecca L.;Zheng, Jie;Brown, Eric W.
通讯作者：
Brown, Eric W.