Genomic characterization of Listeria monocytogenes and Listeria innocua isolated from milk and dairy samples in Ethiopia.
Genomic characterization of Listeria monocytogenes and Listeria innocua isolated from milk and dairy samples in Ethiopia.
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DOI：
10.1186/s12863-024-01195-0
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发表时间：
2024-01-31
期刊：
影响因子：
1.9
通讯作者：
中科院分区：
文献类型：
--
作者：
由单核细胞增生李斯特菌引起的李斯特菌病通常对弱势群体构成重大威胁。乳制品与全球李斯特菌病的爆发有关。在埃塞俄比亚，研究发现了李斯特菌属。和单核细胞增生李斯特氏菌存在于各种乳制品中，但这些细菌的遗传多样性和系统发育关系在低收入和中等收入国家仍然很大程度上未知。因此，我们对从埃塞俄比亚三个地区乳制品供应链不同层面获得的 15 个单增李斯特菌和 55 个无害李斯特菌分离株进行了全基因组测序。基因组被组装并用于 MLST 基因分型和单核苷酸多态性 (SNP) 分析，以推断系统发育关系。我们总共鉴定出 3 个单核细胞增生李斯特菌（即 2、145 和 18）和 12 个无害李斯特菌（即 1489、1619、603、537、1010、3186、492、3007、1087、474、1008 和 637）所研究分离株中的 MLST 序列类型。其中一些序列类型显示区域特异性发生，而其他序列类型则广泛分布于各个区域。通过高质量的SNP分析，我们发现在鉴定为ST 2的13个单增李斯特菌中，有11个高度相似且遗传变异较低，仅相差1至10个SNP，这表明乳制品供应链中存在潜在的选择。 L. innocua 分离株还表现出较低的 ST 内遗传变异，只有 0-10 个 SNP 差异，但 ST 1619 除外，它表现出更大的多样性。在线版本包含可在 10.1186/s12863-024-01195-0 获取的补充材料。
Listeriosis caused by Listeria monocytogenes often poses a significant threat to vulnerable populations. Dairy products have been implicated in outbreaks of listeriosis worldwide. In Ethiopia, studies have identified Listeria spp. and L. monocytogenes in various dairy products, but the genetic diversity and phylogenetic relationships of these bacteria remain largely unknown in the low- and middle-income countries. Therefore, we conducted whole-genome sequencing on 15 L. monocytogenes and 55 L. innocua isolates obtained from different levels of the dairy supply chains across three regions in Ethiopia. Genomes were assembled and used for MLST genotyping and single nucleotide polymorphism (SNP) analysis to infer phylogenetic relationships. We identified a total of 3 L. monocytogenes (i.e., 2, 145, and 18) and 12 L. innocua (i.e., 1489, 1619, 603, 537, 1010, 3186, 492, 3007, 1087, 474, 1008, and 637) MLST sequence types among the studied isolates. Some of these sequence types showed region-specific occurrence, while others were broadly distributed across regions. Through high-quality SNP analysis, we found that among 13 L. monocytogenes identified as ST 2, 11 of them were highly similar with low genetic variation, differing by only 1 to 10 SNPs, suggesting potential selection in the dairy food supply chain. The L. innocua isolates also exhibited low intra-ST genetic variation with only 0–10 SNP differences, except for the ST 1619, which displayed a greater diversity. The online version contains supplementary material available at 10.1186/s12863-024-01195-0.
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影响因子：
2
作者：
Gaulin, Colette;Ramsay, Danielle;Bekal, Sadjia
通讯作者：
Bekal, Sadjia
影响因子：
5.8
作者：
Page, Andrew J.;Cummins, Carla A.;Parkhill, Julian
通讯作者：
Parkhill, Julian
DOI：
10.1590/s1984-82502014000200013
发表时间：
2014-06-01
影响因子：
1.3
作者：
Oltramari, Karine;Cardoso, Rosilene Fressati;Mikcha, Jane Martha Graton
通讯作者：
Mikcha, Jane Martha Graton
影响因子：
2
作者：
PICCININ, DM;SHELEF, LA
通讯作者：
SHELEF, LA
影响因子：
1.7
作者：
Bankevich, Anton;Nurk, Sergey;Pevzner, Pavel A.
通讯作者：
Pevzner, Pavel A.