Effects on growth and sporulation of inactivation of a Bacillus subtilis gene (ctc) transcribed in vitro by minor vegetative cell RNA polymerases (E-σ37, E-σ32)
Effects on growth and sporulation of inactivation of a Bacillus subtilis gene (ctc) transcribed in vitro by minor vegetative cell RNA polymerases (E-σ37, E-σ32)
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小型营养细胞 RNA 聚合酶（E-σ37、E-σ32）体外转录的枯草芽孢杆菌基因 (ctc) 失活对生长和孢子形成的影响
DOI：
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发表时间：
1988
期刊：
影响因子：
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通讯作者：
W. Haldenwang
中科院分区：
文献类型：
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作者：
C. L. Truitt;E. Weaver;W. Haldenwang
通过将E-σ37基因ctc定点插入枯草芽孢杆菌染色体，使其失活。所产生的突变在高温（48° C）下抑制孢子形成达95%。如果将ctc-突变置于一个在紧密相连但不同的spoVC基因中携带突变的菌株中，ctc现在会影响高温下的生长和孢子形成。当指数生长的培养物从37° C转移到48° C时，ctc-spoVC 285菌株的生长被短暂抑制。在B中也观察到类似但不太明显的“生长滞后”。仅携带spoVC-285突变的枯草杆菌菌株。这一发现表明ctc和spoVC产物在植物生长B中均起作用。枯草杆菌。
SummaryThe E-σ37 gene ctc was inactivated by a site-specific insertion into the Bacillus subtilis chromosome. The resulting mutation inhibited sporulation by 95% at elevated temperatures (48° C). If the ctc- mutation is placed in a strain that carries a mutation in the closely linked but distinct spoVC gene, ctc now affects both growth and sporulation at elevated temperatures. Growth of the ctc- spoVC285 strain was transiently inhibited when exponentially growing cultures were shifted from 37° C to 48° C. A similar, but less pronounced “growth lag”, was also seen in a B. subtilis strain carrying only the spoVC-285 mutation. This finding suggests that both the ctc and spoVC products function in vegetatively growing B. subtilis.
DOI：
10.1073/pnas.82.24.8453
发表时间：
1985-12-01
影响因子：
11.1
作者：
HUNT, TP;MAGASANIK, B
通讯作者：
MAGASANIK, B