Preparation of coaxial-electrospun poly[bis(p-methylphenoxy)]phosphazene nanofiber membrane for enzyme immobilization.
Preparation of coaxial-electrospun poly[bis(p-methylphenoxy)]phosphazene nanofiber membrane for enzyme immobilization.
复制标题
酶固定化同轴电纺聚[双(对甲基苯氧基)]磷腈纳米纤维膜的制备
DOI：
10.3390/ijms131114136
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发表时间：
2012-11-02
影响因子：
5.6
通讯作者：
Huang XJ
中科院分区：
文献类型：
--
作者：
Wang SG;Jiang X;Chen PC;Yu AG;Huang XJ
采用同轴静电纺丝技术制备了以聚双（对甲基苯氧基）磷腈（PMPPh）为鞘、聚丙烯腈（PAN）为芯的核/鞘型复合膜。用场发射扫描电子显微镜和透射电子显微镜对膜的形貌进行了表征。结果表明，PAN纺丝液浓度和芯鞘液流量比对同轴静电纺丝过程起决定性作用。此外，稳定的芯/鞘PMPPh固定化膜作为酶固定化的支持，因为它具有良好的生物相容性，高的表面积/体积比，和大的孔隙率进行了研究。采用吸附法将脂肪酶固定化在膜上。研究了聚磷腈膜固定化脂肪酶的性能，并与聚丙烯腈膜进行了比较。结果表明，聚磷腈膜固定化脂肪酶的吸附容量（20.4 ± 2.7 mg/g）和活力保持率（63.7%）均高于PAN膜。
A core/sheath nanofiber membrane with poly[bis(p-methylphenoxy)]phosphazene (PMPPh) as the sheath and easily spinnable polyacrylonitrile (PAN) as the core was prepared via a coaxial electrospinning process. Field-emission scanning electron microscopy and transmission electron microscopy were used to characterize the morphology of the nanofiber membrane. It was found that the concentration of the PAN spinning solution and the ratio of the core/sheath solution flow rates played a decisive role in the coaxial electrospinning process. In addition, the stabilized core/sheath PMPPh nanofiber membrane was investigated as a support for enzyme immobilization because of its excellent biocompatibility, high surface/volume ratio, and large porosity. Lipase from Candida rugosa was immobilized on the nanofiber membrane by adsorption. The properties of the immobilized lipase on the polyphosphazene nanofiber membrane were studied and compared with those of a PAN nanofiber membrane. The results showed that the adsorption capacity (20.4 ± 2.7 mg/g) and activity retention (63.7%) of the immobilized lipase on the polyphosphazene nanofiber membrane were higher than those on the PAN membrane.
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影响因子：
5.5
作者：
Chang, Y;Lee, SC;Allcock, HR
通讯作者：
Allcock, HR
影响因子：
6.2
作者：
Cuetos, Anibal;Valenzuela, Maria L.;Carriedo, Gabino A.
通讯作者：
Carriedo, Gabino A.
影响因子：
14
作者：
ALLCOCK, HR;PUCHER, SR;SCOPELIANOS, AG
通讯作者：
SCOPELIANOS, AG
影响因子：
4.6
作者：
ALLCOCK, HR;KUGEL, RL;VALAN, KJ
通讯作者：
VALAN, KJ
影响因子：
4.6
作者：
Huang, Xiao-Jun;Xu, Zhi-Kang;Seta, Patrick
通讯作者：
Seta, Patrick