Nine orders of magnitude dynamic range: picomolar to millimolar concentration measurement in capillary electrophoresis with laser induced fluorescence detection employing cascaded avalanche photodiode photon counters.
Nine orders of magnitude dynamic range: picomolar to millimolar concentration measurement in capillary electrophoresis with laser induced fluorescence detection employing cascaded avalanche photodiode photon counters.
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DOI：
10.1021/ac103374x
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发表时间：
2011-04-01
影响因子：
7.4
通讯作者：
Dovichi, Norman J.
中科院分区：
文献类型：
--
作者：
Dada, Oluwatosin O.;Essaka, David C.;Hindsgaul, Ole;Palcic, Monica M.;Prendergast, Jillian;Schnaar, Ronald L.;Dovichi, Norman J.
毛细管电泳法的动态范围在低浓度时最终受到分子散粒噪声的限制，而在高浓度时则受到浓度引起的谱带展宽的限制。我们报告了一个接近这些基本限制的系统。报道了一种激光诱导荧光探测器，它采用串联的四个光纤分束器的级联来产生一个主信号和四个衰减信号，每个信号由一个单光子计数雪崩光电二极管监测。对来自五个光电二极管的信号进行适当的定标，得到5-羧基-四甲基罗丹明的线性光学校准曲线，从浓度检测下限1 pm到上限1 mm。质量检测下限是向仪器中注入120微克分子(70个分子)。用甚宽动态范围的仪器研究了大鼠小脑单个细胞产生的荧光标记神经节苷脂GM1-TMR的代谢产物。
The dynamic range of capillary electrophoresis analysis is ultimately limited by molecular shot noise at low concentrations and by concentration-induced band broadening at high concentrations. We report a system that approaches these fundamental limits. A laser-induced fluorescence detector is reported that employs a cascade of four fiber-optic beam-splitters connected in series to generate a primary signal and four attenuated signals, each monitored by a single-photon counting avalanche photodiode. Appropriate scaling of the signals from the five photodiodes produces a linear optical calibration curve for 5-carboxyl-tetramethylrhodamine from the concentration detection limit of 1 pM to the upper limit of 1 mM. Mass detection limits are 120 yoctomoles (70 molecules) injected into the instrument. The very-wide dynamic range instrument was used to study the metabolic products of the fluorescently labeled glycosphingolipid GM1-TMR produced by single cells isolated from the rat cerebellum.
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影响因子：
7.4
作者：
Kissick, David J.;Muir, Ryan D.;Simpson, Garth J.
通讯作者：
Simpson, Garth J.
影响因子：
--
作者：
BLACKMAN, M;MICHIELS, JL
通讯作者：
MICHIELS, JL
影响因子：
7.4
作者：
Hu, K;Ahmadzadeh, H;Krylov, SN
通讯作者：
Krylov, SN
影响因子：
7.4
作者：
INGLE, JD;CROUCH, SR
通讯作者：
CROUCH, SR
毛细血管电泳中的六个数量级动态范围，具有超敏激光诱导的荧光检测。
影响因子：
6.1
作者：
Whitmore, Colin D.;Essaka, David;Dovichi, Norman J.
通讯作者：
Dovichi, Norman J.