Six orders of magnitude dynamic range in capillary electrophoresis with ultrasensitive laser-induced fluorescence detection.
Six orders of magnitude dynamic range in capillary electrophoresis with ultrasensitive laser-induced fluorescence detection.
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毛细血管电泳中的六个数量级动态范围，具有超敏激光诱导的荧光检测。
DOI：
10.1016/j.talanta.2009.07.060
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发表时间：
2009-12-15
期刊：
影响因子：
6.1
通讯作者：
Dovichi, Norman J.
中科院分区：
文献类型：
--
作者：
Whitmore, Colin D.;Essaka, David;Dovichi, Norman J.
使用超灵敏激光诱导荧光检测器和毛细管电泳来研究 5-羧基-四甲基罗丹明。来自检测器的原始信号提供了大约三个数量级的动态范围。由于与检测器中使用的单光子计数雪崩光电二极管相关的死区时间，信号在高分析物浓度下饱和。信号可以针对检测器死区时间进行校正，从而提供额外的数量级动态范围。为了进一步增加动态范围，两个光纤分束器级联以生成一个主信号和两个衰减信号，每个信号均由单光子计数雪崩光电二极管监控。来自三个光电二极管的组合信号从浓度检测限 3 pM 到 10 μM（所研究的最大浓度，范围为 3,000,000）呈合理的线性。质量检测限为注入毛细管的 150 yocmoles。
An ultrasensitive laser-induced fluorescence detector was used with capillary electrophoresis for the study of 5-carboxy-tetramethylrhodamine. The raw signal from the detector provided roughly three orders of magnitude dynamic range. The signal saturated at high analyte concentrations due to the dead time associated with the single-photon counting avalanche photodiode employed in the detector. The signal can be corrected for the detector dead time, providing an additional order of magnitude dynamic range. To further increase dynamic range, two fiber-optic beam-splitters were cascaded to generate a primary signal and two attenuated signals, each monitored by a single-photon counting avalanche photodiode. The combined signals from the three photodiodes are reasonably linear from the concentration detection limit of 3 pM to 10 μM, the maximum concentration investigated, a range of 3,000,000. Mass detection limits were 150 yoctomoles injected onto the capillary.
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影响因子：
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作者：
BLACKMAN, M;MICHIELS, JL
通讯作者：
MICHIELS, JL
影响因子：
7.4
作者：
Turner, Emily H.;Lauterbach, Kevin;Dovichi, Norman J.
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