Chloroplastic thioredoxin-f and thioredoxin-m1/4 play important roles in brassinosteroids-induced changes in CO2 assimilation and cellular redox homeostasis in tomato.
Chloroplastic thioredoxin-f and thioredoxin-m1/4 play important roles in brassinosteroids-induced changes in CO2 assimilation and cellular redox homeostasis in tomato.
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DOI：
10.1093/jxb/eru207
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发表时间：
2014-08
影响因子：
6.9
通讯作者：
Yu JQ
中科院分区：
文献类型：
--
作者：
Cheng F;Zhou YH;Xia XJ;Shi K;Zhou J;Yu JQ
本研究利用病毒诱导的基因沉默（VIGS）来研究硫氧还蛋白-f和硫氧还蛋白-m1/4在油菜素内酯诱导的番茄CO2同化和细胞氧化还原稳态变化中的作用。叶绿体硫氧还蛋白（TRXs）和谷胱甘肽作为氧化还原信使在光合作用的调节中发挥作用。本研究以番茄叶片为材料，研究了叶绿体TRXs在油菜素内酯（BR）诱导的细胞氧化还原稳态和CO2同化变化中的作用。BRs缺陷的d ^im植株表现出TRX-f、TRX-m2、TRX-m1/4和TRX-x的转录水平降低，而外源BRs显著诱导CO2同化和TRX-f、TRX-m2、TRX-m1/4和TRX-x的表达。病毒诱导的叶绿体TRX-f、TRX-m2、TRX-m1/4和TRX-y基因沉默（VIGS）分别增加了膜脂过氧化和2-Cys过氧化物氧还蛋白二聚体的积累，降低了叶片中抗坏血酸-谷胱甘肽循环酶的活性和还原型谷胱甘肽与氧化型谷胱甘肽的比值（GSH/GSSG）。此外，TRX-f、TRX-m2、TRX-m1/4和TRX-y的部分沉默导致参与Benson-Calvin循环的基因表达降低，相关酶的活性降低。重要的是，BR诱导的CO2同化增加和抗氧化剂和光合作用相关基因和酶的表达和活性增加在部分TRX-f和TRX-m1/4沉默的植物中受到损害。这些结果表明TRX-f和TRX-m1/4参与了BRs诱导的番茄CO2同化和细胞氧化还原稳态的变化。
Virus-induced gene silencing (VIGS) was used in this study to characterize the role of thioredoxin-f and thioredoxin-m1/4 in brassinosteroid-induced changes in CO2 assimilation and cellular redox homeostasis in tomato. Chloroplast thioredoxins (TRXs) and glutathione function as redox messengers in the regulation of photosynthesis. In this work, the roles of chloroplast TRXs in brassinosteroids (BRs)-induced changes in cellular redox homeostasis and CO2 assimilation were studied in the leaves of tomato plants. BRs-deficient d ^im plants showed decreased transcripts of TRX-f, TRX-m2, TRX-m1/4, and TRX-x, while exogenous BRs significantly induced CO2 assimilation and the expression of TRX-f, TRX-m2, TRX-m1/4, and TRX-x. Virus-induced gene silencing (VIGS) of the chloroplast TRX-f, TRX-m2, TRX-m1/4, and TRX-y genes individually increased membrane lipid peroxidation and accumulation of 2-Cys peroxiredoxin dimers, and decreased the activities of the ascorbate–glutathione cycle enzymes and the ratio of reduced glutathione to oxidized glutathione (GSH/GSSG) in the leaves. Furthermore, partial silencing of TRX-f, TRX-m2, TRX-m1/4, and TRX-y resulted in decreased expression of genes involved in the Benson–Calvin cycle and decreased activity of the associated enzymes. Importantly, the BRs-induced increase in CO2 assimilation and the increased expression and activities of antioxidant- and photosynthesis-related genes and enzymes were compromised in the partially TRX-f- and TRX-m1/4-silenced plants. All of these results suggest that TRX-f and TRX-m1/4 are involved in the BRs-induced changes in CO2 assimilation and cellular redox homeostasis in tomato.
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影响因子：
4.8
作者：
Collin, V;Issakidis-Bourguet, E;Miginiac-Maslow, M
通讯作者：
Miginiac-Maslow, M
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DOI：
10.1016/j.bbrc.2012.08.100
发表时间：
2012-09-28
影响因子：
3.1
作者：
Jiang, Yu Ping;Cheng, Fei;Yu, Jing Quan
通讯作者：
Yu, Jing Quan
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影响因子：
7.3
作者：
Cui, Jin-Xia;Zhou, Yan-Hong;Yu, Jing-Quan
通讯作者：
Yu, Jing-Quan
细胞谷胱甘肽氧化还原稳态在油菜素类固醇诱导的黄瓜 CO2 同化增加中发挥重要作用
影响因子：
9.4
作者：
Jiang, Yu-Ping;Cheng, Fei;Yu, Jing-Quan
通讯作者：
Yu, Jing-Quan
影响因子：
5.8
作者：
Jacquot, JP;Gelhaye, E;Aubry, A
通讯作者：
Aubry, A