Two calmodulins in Naegleria flagellates: characterization, intracellular segregation, and programmed regulation of mRNA abundance during differentiation.
Two calmodulins in Naegleria flagellates: characterization, intracellular segregation, and programmed regulation of mRNA abundance during differentiation.
复制标题
Naegleria鞭毛中的两个钙调蛋白：在分化过程中对mRNA丰度的表征，细胞内分离和编程的调节。
DOI：
10.1083/jcb.102.5.1671
复制
发表时间：
1986-05
影响因子：
7.8
通讯作者：
LAI, EY
中科院分区：
文献类型：
--
作者：
FULTON, C;CHEN, KL;LAI, EY
Naegleria gruberi的鞭毛含有两种钙调素，其表观分子量和细胞内位置不同。位于鞭毛中的钙调素-1具有约16，000的表观分子量，其大小与其他原生动物钙调素的大小大致相同，而位于细胞体中的钙调素-2为15，300。这两种蛋白质，纯化，钙调素的几个标准，包括钙调素依赖性环核苷酸磷酸二酯酶和抗体的脊椎动物钙调素的亲和力的钙调素依赖性刺激。两种钙调素的发现是不寻常的。由于唯一已知的差异是表观分子量，一种钙调素可以从另一种衍生，除了两种钙调素都是在小麦胚芽中合成的，无细胞系统由分化的耐格里属的RNA指导。可翻译的mRNA编码钙调素1和2，未检测到阿米巴，出现，随后消失的同时在100分钟的分化Naegleria从阿米巴鞭毛虫。此外，这些mRNA的丰度增加，然后减少与鞭毛微管蛋白，这表明钙调蛋白和微管蛋白的无关基因的表达可能是在分化过程中协调控制的可能性。
Flagellates of Naegleria gruberi contain two calmodulins that differ in apparent molecular weight and intracellular location. Calmodulin-1, localized in flagella, has an apparent molecular weight of approximately 16,000, approximately the size of other protozoan calmodulins, whereas calmodulin-2, localized in cell bodies, is 15,300. Both proteins, purified, are calmodulins by several criteria, including Ca2+-dependent stimulation of calmodulin-dependent cyclic nucleotide phosphodiesterase and affinity for antibodies to vertebrate calmodulin. The finding of two calmodulins is unusual. Since the only known difference is apparent molecular weight, one calmodulin could be derived from the other, except that both calmodulins are synthesized in a wheat germ, cell-free system directed by RNA from differentiating Naegleria. Translatable mRNAs encoding calmodulins 1 and 2, not detected in amebas, appear and subsequently disappear concurrently during the 100-min differentiation of Naegleria from amebas to flagellates. Furthermore, these mRNAs increase and then decrease in abundance concurrently with those for flagellar tubulins, which suggests the possibility that the expression of the unrelated genes for calmodulin and tubulin may be under coordinate control during differentiation.
登录
查看更多内容
影响因子：
5.3
作者：
CHIEN, YH;DAWID, IB
通讯作者：
DAWID, IB
DOI：
10.1016/0006-291x(82)90712-4
发表时间：
1982-01-01
影响因子：
3.1
作者：
GOPALAKRISHNA, R;ANDERSON, WB
通讯作者：
ANDERSON, WB
影响因子：
64.5
作者：
LAI, EY;WALSH, C;FULTON, C
通讯作者：
FULTON, C
DOI：
10.1083/jcb.85.2.346
发表时间：
1980-05
期刊：
The Journal of cell biology
影响因子：
--
作者：
Fulton C;Walsh C
通讯作者：
Walsh C
DOI：
10.1016/0005-2795(80)90254-8
发表时间：
1980-01-01
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
BURGESS, WH;JEMIOLO, DK;KRETSINGER, RH
通讯作者：
KRETSINGER, RH