Chemical and structural analysis of Eucalyptus globulus and E. camaldulensis leaf cuticles: a lipidized cell wall region.
Chemical and structural analysis of Eucalyptus globulus and E. camaldulensis leaf cuticles: a lipidized cell wall region.
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DOI：
10.3389/fpls.2014.00481
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发表时间：
2014
影响因子：
5.6
通讯作者：
Gil L
中科院分区：
文献类型：
--
作者：
Guzmán P;Fernández V;Graça J;Cabral V;Kayali N;Khayet M;Gil L
传统上，植物角质层被认为是覆盖在外表皮细胞壁上的一个独立的疏水层。由于角质层的复杂性，目前角质层化学成分与超微结构之间的关系尚不清楚。本研究旨在通过逐步提取和鉴定脂类成分(角质和可溶性脂类)，结合光谱分析和显微分析，研究赤桉和蓝桉分离的正面叶角质层的化学成分和结构之间的联系。鉴定出的可溶性化合物和角质蛋白单体不能归属于具体的内部角质层超微结构。角质解聚后，观察到与细胞壁相似的纤维素网，其结构模式不同，分别归属于角质层和角质层。我们的结果表明，目前的角质层模型应该进行修改，强调细胞壁多糖的存在和主要作用。因此，角质层可能被解释为含有额外脂类的修饰细胞壁区域。植物角质层的主要异质性使得很难用现有的方法在角质层化学和结构之间建立直接的联系。
The plant cuticle has traditionally been conceived as an independent hydrophobic layer that covers the external epidermal cell wall. Due to its complexity, the existing relationship between cuticle chemical composition and ultra-structure remains unclear to date. This study aimed to examine the link between chemical composition and structure of isolated, adaxial leaf cuticles of Eucalyptus camaldulensis and E. globulus by the gradual extraction and identification of lipid constituents (cutin and soluble lipids), coupled to spectroscopic and microscopic analyses. The soluble compounds and cutin monomers identified could not be assigned to a concrete internal cuticle ultra-structure. After cutin depolymerization, a cellulose network resembling the cell wall was observed, with different structural patterns in the regions ascribed to the cuticle proper and cuticular layer, respectively. Our results suggest that the current cuticle model should be revised, stressing the presence and major role of cell wall polysaccharides. It is concluded that the cuticle may be interpreted as a modified cell wall region which contains additional lipids. The major heterogeneity of the plant cuticle makes it difficult to establish a direct link between cuticle chemistry and structure with the existing methodologies.
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影响因子：
4
作者：
Heredia-Guerrero, Jose A.;Benitez, Jose J.;Heredia, Antonio
通讯作者：
Heredia, Antonio
影响因子：
3
作者：
Deshmukh, AP;Simpson, AJ;Hatcher, PG
通讯作者：
Hatcher, PG
影响因子：
5.6
作者：
Fernández V;Brown PH
通讯作者：
Brown PH
影响因子：
6.5
作者：
Guzman, Paula;Fernandez, Victoria;Gil, Luis
通讯作者：
Gil, Luis
影响因子：
6.1
作者：
Belge, Burcu;Llovera, Montserrat;Lara, Isabel
通讯作者：
Lara, Isabel