Pathogenic Process-Associated Transcriptome Analysis of Stemphylium lycopersici from Tomato.
Pathogenic Process-Associated Transcriptome Analysis of Stemphylium lycopersici from Tomato.
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DOI：
10.1155/2022/4522132
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发表时间：
2022
影响因子：
2.9
通讯作者：
Li, Fajun
中科院分区：
文献类型：
--
作者：
Zhang, Dezhen;Chi, Wenjuan;Wang, Cuicui;Dai, Huijie;Li, Jintang;Li, Chunlei;Li, Fajun
番茄灰斑病是我国番茄上的一种主要病害，主要由番茄茎腐病病原菌引起。关于这种广泛宿主范围的病原体的致病机制知之甚少。本研究通过比较转录组学分析，获得了更多的致病性决定因子的遗传信息。结果表明，番茄枯萎病菌侵染番茄的过程中，通过RNA测序（RNA-seq）分析，分别在早期感染和坏死营养阶段期间上调的1，642和1，875个基因被鉴定，并分别在44和24个途径中显著富集。与果胶降解，粘附和定植相关的基因的诱导是显着的，在感染早期阶段，而在necrotrophic阶段，一些结构分子活性相关的基因显着诱导。此外，一些参与信号调节或编码半纤维素和纤维素降解酶和胞外蛋白酶的基因通常在发病过程中上调。总的来说，我们提出了一些可能对S。番茄致病机理在寄主表面的粘附和定殖能力，有效地破坏寄主细胞壁，调节信号转导以管理感染，以及在不利的植物环境中的生存能力被认为是致病的重要因素。番茄中的番茄。这些基因的功能特性为分析这一重要的致病系统提供了宝贵的资源。番茄和番茄，它可能有助于对这种毁灭性的疾病的控制策略的产生。
Tomato (Solanum lycopersicum) gray leaf spot disease is a predominant foliar disease of tomato in China that is caused mainly by the necrotrophic fungal pathogen Stemphylium lycopersici. Little is known regarding the pathogenic mechanisms of this broad-host-range pathogen. In this study, a comparative transcriptomic analysis was performed and more genetic information on the pathogenicity determinants of S. lycopersici during the infection process in tomato were obtained. Through an RNA sequencing (RNA-seq) analysis, 1,642 and 1,875 genes upregulated during the early infection and necrotrophic phases, respectively, were identified and significantly enriched in 44 and 24 pathways, respectively. The induction of genes associated with pectin degradation, adhesion, and colonization was notable during the early infection phase, whereas during the necrotrophic phase, some structural molecule activity-related genes were prominently induced. Additionally, some genes involved in signal regulation or encoding hemicellulose- and cellulose-degrading enzymes and extracellular proteases were commonly upregulated during pathogenesis. Overall, we present some putative key genes and processes that may be crucial for S. lycopersici pathogenesis. The abilities to adhere and colonize a host surface, effectively damage host cell walls, regulate signal transduction to manage infection, and survive in a hostile plant environment are proposed as important factors for the pathogenesis of S. lycopersici in tomato. The functional characterization of these genes provides an invaluable resource for analyses of this important pathosystem between S. lycopersici and tomato, and it may facilitate the generation of control strategies against this devastating disease.
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影响因子：
6.7
作者：
Fu, Huilan;Chung, Kuang-Ren;Li, Hongye
通讯作者：
Li, Hongye
影响因子：
1.9
作者：
Cho, HJ;Kim, BS;Hwang, HS
通讯作者：
Hwang, HS
影响因子：
4.4
作者：
JONES, MJ;EPSTEIN, L
通讯作者：
EPSTEIN, L
影响因子：
4.9
作者：
Leroch, Michaela;Mueller, Nathalie;Hahn, Matthias
通讯作者：
Hahn, Matthias
DOI：
10.1590/s0100-41582005000300002
发表时间：
2005-05-01
期刊：
Fitopatologia Brasileira
影响因子：
--
作者：
Bentes, Jania L. S.;Matsuoka, Kiyoshi
通讯作者：
Matsuoka, Kiyoshi