Clinical collection and protein properties of expressed prostatic secretions as a source for biomarkers of prostatic disease.
Clinical collection and protein properties of expressed prostatic secretions as a source for biomarkers of prostatic disease.
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DOI：
10.1016/j.jprot.2009.01.007
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发表时间：
2009-08-20
影响因子：
3.3
通讯作者：
Semmes, O. John
中科院分区：
文献类型：
--
作者：
Drake, Richard R.;White, Krista Y.;Fuller, Thomas W.;Igwe, Elena;Clements, Mary Ann;Nyalwidhe, Julius O.;Given, Robert W.;Lance, Raymond S.;Semmes, O. John
前列腺会在前列腺液中分泌许多蛋白质，这种前列腺液与精囊液结合在一起，可以促进精子的激活和功能。临床上可以收集的前列腺近端液有精浆和前列腺液。EPS代表前列腺在接受直肠指压按摩后分泌的液体，而这些液体又可以在检查后排出的尿液中收集。这种收集不会扰乱标准的泌尿学检查，而且可以从所有前列腺疾病州的男性身上重复收集。在前列腺切除术前，也可以在麻醉下直接收集EPS液。虽然人们正在为EPS尿液中脱落的上皮细胞部分开发多种前列腺癌检测基因检测方法，但含有许多前列腺癌衍生蛋白的剩余液体的特征已经达到了最低限度。描述了符合当前泌尿学检查和外科实践的EPS收集的优化和标准化的方法，并总结了前列腺特异性抗原和前列腺酸性磷酸酶对EPS液中前列腺特异性抗原和前列腺酸性磷酸酶的初步蛋白质组和糖学评估。EPS尿中前列腺特异性蛋白成分的持续特征，结合临床收集程序的优化，应有助于发现前列腺癌的新生物标记物。
The prostate gland secretes many proteins in a prostatic fluid that combines with seminal vesicle derived fluids to promote sperm activation and function. Proximal fluids of the prostate that can be collected clinically are seminal plasma and expressed-prostatic secretion (EPS) fluids. EPS represents the fluid being secreted by the prostate following a digital rectal prostate massage, which in turn can be collected in voided urine post-exam. This collection is not disruptive to a standard urological exam, and it can be repeatedly collected from men across all prostatic disease states. A direct EPS fluid can also be collected under anesthesia prior to prostatectomy. While multiple genetic assays for prostate cancer detection are being developed for the shed epithelial cell fraction of EPS urines, the remaining fluid that contains many prostate-derived proteins has been minimally characterized. Approaches to optimization and standardization of EPS collection consistent with current urological exam and surgical practices are described, and initial proteomic and glycomic evaluations of the of EPS fluid are summarized for prostate specific antigen and prostatic acid phosphatase. Continued characterization of the prostate specific protein components of EPS urine combined with optimization of clinical collection procedures should facilitate discovery of new biomarkers for prostate cancer.
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影响因子：
6.6
作者：
Deras, Ina L.;Aubin, Sheila M. J.;Groskopf, Jack
通讯作者：
Groskopf, Jack
影响因子：
11.5
作者：
Gross, Mitchell;Top, Irina;Agus, David
通讯作者：
Agus, David
影响因子：
2.8
作者：
Jankovic, MM;Kosanovic, MM
通讯作者：
Kosanovic, MM
DOI：
10.1046/j.0014-2956.2001.02667.x
发表时间：
2002-01-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
Brillard-Bourdet, M;Réhault, S;Gauthier, F
通讯作者：
Gauthier, F
影响因子：
--
作者：
Ceroni A;Dell A;Haslam SM
通讯作者：
Haslam SM