Fluorescent Gold Nanoclusters for Selective Detection of Dopamine in Cerebrospinal fluid.
Fluorescent Gold Nanoclusters for Selective Detection of Dopamine in Cerebrospinal fluid.
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DOI：
10.1038/srep40298
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发表时间：
2017-01-09
影响因子：
4.6
通讯作者：
Yun K
中科院分区：
文献类型：
--
作者：
Govindaraju S;Ankireddy SR;Viswanath B;Kim J;Yun K
近二十年来，蛋白质共轭荧光金纳米团簇(NCS)以其优异的光稳定性在医学和纳米生物技术领域引起了极大的关注。本文报道了一种稳定、无毒、红色荧光发射的牛血清白蛋白-金纳米管选择性检测脑脊液中的多巴胺(DA)。用紫外可见光谱、高分辨电子显微镜、X射线光电子能谱、X射线衍射仪、傅立叶变换红外光谱、光致发光光谱等手段对其演化过程进行了研究。所合成的牛血清白蛋白-金纳米管的荧光强度为4-6 nm，荧光量子产率(QY)为8%。加入不同浓度的DA后，BSA-Au纳米管的荧光强度通过电子转移机制被猝灭。牛血清白蛋白-金纳米管荧光强度的降低使测定缓冲液中DA和脑脊液中DA的线性范围分别为0.622~10 nM和0.830 nM。据我们所知，所制备的BSA-Au NCs将为生物传感器、药物发现以及帕金森氏症和阿尔茨海默病等疾病的快速诊断提供可能的策略和良好的平台。
Since the last two decades, protein conjugated fluorescent gold nanoclusters (NCs) owe much attention in the field of medical and nanobiotechnology due to their excellent photo stability characteristics. In this paper, we reported stable, nontoxic and red fluorescent emission BSA-Au NCs for selective detection of L-dopamine (DA) in cerebrospinal fluid (CSF). The evolution was probed by various instrumental techniques such as UV-vis spectroscopy, High resolution transmission electron microscopy (HTEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), photoluminescence spectroscopy (PL). The synthesised BSA-Au NCs were showing 4–6 nm with high fluorescent ~8% Quantum yield (QY). The fluorescence intensity of BSA-Au NCs was quenched upon the addition of various concentrations of DA via an electron transfer mechanism. The decrease in BSA-Au NCs fluorescence intensity made it possible to determine DA in PBS buffer and the spiked DA in CSF in the linear range from 0 to 10 nM with the limit of detection (LOD) 0.622 and 0.830 nM respectively. Best of our knowledge, as-prepared BSA-Au NCs will gain possible strategy and good platform for biosensor, drug discovery, and rapid disease diagnosis such as Parkinson’s and Alzheimer diseases.
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DOI：
10.3390/molecules21050618
发表时间：
2016-05-11
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Li D;Ao K;Wang Q;Lv P;Wei Q
通讯作者：
Wei Q
影响因子：
7.4
作者：
Rees, Hillary R.;Anderson, Sean E.;Privman, Eve;Bau, Haim H.;Venton, B. Jill
通讯作者：
Venton, B. Jill
影响因子：
8.4
作者：
Adekunle, Abolanle S.;Agboola, Bolade O.;Ozoemena, Kenneth I.
通讯作者：
Ozoemena, Kenneth I.
影响因子：
41.2
作者：
Cho, Eun Seon;Kim, Jiwon;Grzybowski, Bartosz A.
通讯作者：
Grzybowski, Bartosz A.
影响因子：
8.4
作者：
Hou, Jiagang;Xu, Caixia;Zhou, Jianhua
通讯作者：
Zhou, Jianhua