Carbon nanopipette electrodes for dopamine detection in Drosophila.
Carbon nanopipette electrodes for dopamine detection in Drosophila.
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DOI：
10.1021/ac504596y
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发表时间：
2015-04-07
影响因子：
7.4
通讯作者：
Venton, B. Jill
中科院分区：
文献类型：
--
作者：
Rees, Hillary R.;Anderson, Sean E.;Privman, Eve;Bau, Haim H.;Venton, B. Jill
体内神经递质测量需要小型、坚固、灵敏的电极。碳纳米移液器之前已制造并用于单细胞药物输送和电生理学测量。在这里，开发了一种改进的制造程序来生产批量的固体碳纳米移液管电极（CNPE），其尖端直径约为 250 nm，暴露碳的长度可控，范围从 5 μm 到 175 μm。首次采用快速扫描循环伏安法（FSCV）对 CNPE 的电化学性能进行了表征。 CNPE 用于检测电活性神经递质多巴胺、血清素和章鱼胺。 CNPE 对血清素检测比传统碳纤维微电极 (CFME) 更加敏感。与 CFME 类似，CNPE 对多巴胺浓度范围为 0.1 至 10 μM 且 LOD 为 25 ± 5 nM 具有线性响应。当所施加的三角波形相对于 Ag/AgCl/Cl− 以 400 V/s 的速度在 -0.4 和 1.3 V 之间扫描时，CNPE 的记录可以稳定超过 3 小时。 CNPEs被用来利用光遗传学检测果蝇幼虫内源性多巴胺的释放，这验证了CNPEs在体内神经科学研究中的实用性。 CNPE 具有优势，因为它们的直径比典型的 CFME 小一个数量级，并且具有尖锐、可调的几何形状，有助于穿透和植入，以便在小型生物体的不同区域（例如果蝇大脑）进行局部测量。
Small, robust, sensitive electrodes are desired for in vivo neurotransmitter measurements. Carbon nanopipettes have been previously manufactured and used for single cell drug delivery and electrophysiological measurements. Here, a modified fabrication procedure was developed to produce batches of solid carbon nanopipette electrodes (CNPEs) with ~250 nm diameter tips, and controllable lengths of exposed carbon, ranging from 5 μm to 175 μm. The electrochemical properties of CNPEs were characterized with fast-scan cyclic voltammetry (FSCV) for the first time. CNPEs were used to detect the electroactive neurotransmitters dopamine, serotonin, and octopamine. CNPEs were significantly more sensitive for serotonin detection than traditional carbon fiber microelectrodes (CFMEs). Similar to CFMEs, CNPEs have a linear response for dopamine concentrations ranging from 0.1 to 10 μM and a LOD of 25 ± 5 nM. Recordings with CNPEs were stable for over 3 hours when the applied triangle waveform was scanned between −0.4 and 1.3 V vs. Ag/AgCl/Cl− at 400 V/s. CNPEs were used to detect endogenous dopamine release in Drosophila larvae using optogenetics, which verified the utility of CNPEs for in vivo neuroscience studies. CNPEs are advantageous because they are an order of magnitude smaller in diameter than typical CFMEs and have a sharp, tunable geometry that facilitates penetration and implantation for localized measurements in distinct regions of small organisms, such as the Drosophila brain.
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影响因子：
4.2
作者：
Heien, MLAV;Phillips, PEM;Wightman, RM
通讯作者：
Wightman, RM
影响因子：
7.4
作者：
Bath, BD;Michael, DJ;Wightman, RM
通讯作者：
Wightman, RM
一种低噪声碳纤维纳米电极的制备方法
影响因子：
7.4
作者：
Huang, WH;Pang, DW;Cheng, JK
通讯作者：
Cheng, JK
影响因子：
3
作者：
Chadchankar, Heramb;Yavich, Leonid
通讯作者：
Yavich, Leonid
DOI：
10.1039/c1an15025a
发表时间：
2011-05-07
期刊：
The Analyst
影响因子：
--
作者：
Koehne JE;Marsh M;Boakye A;Douglas B;Kim IY;Chang SY;Jang DP;Bennet KE;Kimble C;Andrews R;Meyyappan M;Lee KH
通讯作者：
Lee KH