Structure of dehydroergosterol monohydrate and interaction with sterol carrier protein-2.
Structure of dehydroergosterol monohydrate and interaction with sterol carrier protein-2.
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DOI：
10.1007/s11745-008-3267-1
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发表时间：
2008-12
期刊：
影响因子：
1.9
通讯作者：
Schroeder, Friedhelm
中科院分区：
文献类型：
--
作者：
McIntosh, Avery L.;Atshaves, Barbara P.;Gallegos, Adalberto M.;Storey, Stephen M.;Reibenspies, Joseph H.;Kier, Ann B.;Meyer, Edgar;Schroeder, Friedhelm
脱氢麦角甾醇[ergosta-5，7，9(11)，22-四烯-3β-ol]是一种天然存在的荧光甾醇，在体内外被广泛用于探测膜胆固醇的分布、胆固醇与蛋白质的相互作用以及胆固醇的细胞内转运。在水溶液中，脱氢麦角甾醇的低溶解度导致形成类似于胆固醇的一水晶体。低温X射线衍射分析表明，脱氢麦角甾醇一水合物属于P21空间群，晶胞中有4个分子，单斜晶胞参数a=9.975(1)ä，b=7.4731(9)ä，c=34.054(4)ä，β=92.970(2)°，与一水麦角甾醇有些相似。分子排列在一个稍微紧密堆积的双层结构中，类似于一水胆固醇。由于脱氢麦角甾醇的荧光发射在结晶时会经历量子产率的提高和最大波长的红移，因此利用基于试管的光谱和多光子激光扫描成像显微镜(MPLSM)可以高灵敏度地监测微晶的形成或破坏。本文报道了脱氢麦角甾醇一水微晶供体和受体的水分散体之间的相互作用的动力学效应。受体既包括模型膜，也包括通过生化分离和亲和纯化得到的质膜上的囊泡。此外，这项研究还提供了SCP-2水平升高对活细胞中脱氢麦角固醇微晶消失率的影响的实时测量。
Dehydroergosterol [ergosta-5,7,9(11),22-tetraen-3β-ol] is a naturally-occurring, fluorescent sterol utilized extensively to probe membrane cholesterol distribution, cholesterol-protein interactions, and intracellular cholesterol transport both in vitro and in vivo. In aqueous solutions, the low solubility of dehydroergosterol results in the formation of monohydrate crystals similar to cholesterol. Low temperature x-ray diffraction analysis reveals that dehydroergosterol monohydrate crystallizes in the space group P21 with 4 molecules in the unit cell and monoclinic crystal parameters a = 9.975(1)Å, b = 7.4731(9)Å, c = 34.054(4)Å, and β = 92.970(2)° somewhat similar to ergosterol monohydrate. The molecular arrangement is in a slightly closer packed bilayer structure resembling cholesterol monohydrate. Since dehydroergosterol fluorescence emission undergoes a quantum yield enhancement and red-shift of its maximum wavelength when crystallized, formation or disruption of microcrystals was monitored with high sensitivity using cuvette-based spectroscopy and multi-photon laser scanning imaging microscopy (MPLSM). This manuscript reports on the dynamical effect of sterol carrier protein-2 (SCP-2) interacting between aqueous dispersions of dehydroergosterol monohydrate microcrystal donors and acceptors consisting not only of model membranes but also vesicles derived from plasma membranes isolated by biochemical fractionation and affinity purification from Madin-Darby canine kidney cells. Furthermore, this study provides real-time measurements of the effect of increased SCP-2 levels on the rate of disappearance of dehydroergosterol microcrystals in living cells.
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影响因子：
4.8
作者：
Frolov, A;Cho, TH;Schroeder, F
通讯作者：
Schroeder, F
DOI：
10.1083/jcb.200211018
发表时间：
2003-02-03
期刊：
The Journal of cell biology
影响因子：
--
作者：
Abrami L;Liu S;Cosson P;Leppla SH;van der Goot FG
通讯作者：
van der Goot FG
影响因子：
1.8
作者：
DELSETH, C;KASHMAN, Y;DJERASSI, C
通讯作者：
DJERASSI, C
影响因子：
2.9
作者：
BERGERON, R;SCOTT, J
通讯作者：
SCOTT, J
DOI：
10.1016/0165-022x(92)90043-a
发表时间：
1992-03-01
影响因子：
--
作者：
BUTKO, P;HAPALA, I;SCHROEDER, F
通讯作者：
SCHROEDER, F